纳摩尔级天然产物的核磁共振定量分析。
NMR quantitation of natural products at the nanomole scale.
作者信息
Dalisay Doralyn S, Molinski Tadeusz F
机构信息
Department of Chemistry and Biochemistry and Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, California 92093-0358, USA.
出版信息
J Nat Prod. 2009 Apr;72(4):739-44. doi: 10.1021/np900009b.
Abstract
We describe a simple and accurate method for quantitation by solvent 13C-satellites (QSCS) of very small amounts of natural products using microprobe NMR spectroscopy. The method takes advantage of integration of 13C satellite peaks of deuterated solvents, in particular CDCl3, that have favorable intensities for measurements of samples in NMR microcoils and microprobe tubes in the 1-200 nanomole range.
摘要
我们描述了一种利用微探针核磁共振波谱法通过溶剂13C卫星峰（QSCS）对极少量天然产物进行定量的简单且准确的方法。该方法利用了氘代溶剂（特别是CDCl3）的13C卫星峰积分，这些峰具有适宜的强度，可用于在核磁共振微线圈和微探针管中对1-200纳摩尔范围内的样品进行测量。
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