Ge Jingyan, Wang Yinan, Feng Ye, Liu Haiyan, Cui Xueling, Chen Fangfang, Tai Guixiang, Liu Zhonghui
Department of Immunology, Norman Bethune College of Medicine, Jilin University, Changchun 130021, China.
Cell Mol Immunol. 2009 Apr;6(2):129-33. doi: 10.1038/cmi.2009.18.
Macrophages play critical roles in innate immune and acquired immune via secreting pro-inflammatory mediators, phagocytosing microorganisms and presenting antigens. Activin A, a member of transforming growth factor beta (TGF-beta) superfamily, is produced by macrophages and microglia cells. In this study, we reported a direct effect of activin A as a pro-inflammatory factor on mouse macrophage cell line RAW264.7 cells. Our data revealed that activin A could not only increase IL-1 beta and IL-6 production from RAW264.7 cells, but also promote pinocytic and phagocytic activities of RAW264.7 cells. In addition, activin A obviously up-regulated MHC II expression on the surface of RAW264.7 cells, whereas did not influence MHC I expression. Activin A also enhanced CD80 expression, which is a marker of activated macrophages, but did not influence RAW264.7 cell proliferation. These data suggest that activin A may regulate primary macrophage-mediated innate and acquired immune response via promoting the activation of rest macrophages.
巨噬细胞通过分泌促炎介质、吞噬微生物和呈递抗原在天然免疫和获得性免疫中发挥关键作用。激活素A是转化生长因子β（TGF-β）超家族的成员，由巨噬细胞和小胶质细胞产生。在本研究中，我们报道了激活素A作为一种促炎因子对小鼠巨噬细胞系RAW264.7细胞的直接作用。我们的数据显示，激活素A不仅能增加RAW264.7细胞中白细胞介素-1β和白细胞介素-6的产生，还能促进RAW264.7细胞的胞饮和吞噬活性。此外，激活素A明显上调RAW264.7细胞表面主要组织相容性复合体II（MHC II）的表达，而不影响MHC I的表达。激活素A还增强了作为活化巨噬细胞标志物的CD80的表达，但不影响RAW264.7细胞的增殖。这些数据表明，激活素A可能通过促进静止巨噬细胞的活化来调节初级巨噬细胞介导的天然免疫和获得性免疫反应。
