This study examined the maturation pattern of fatigue resistance (FR) from childhood to adulthood in females and males during high-intensity intermittent exercise and compared FR between females and males in childhood and adolescence. Thirty males (boys 11.3 +/- 0.5 years, teen-males 14.7 +/- 0.3 years, men 24.0 +/- 2.1 years) and 30 females (girls 10.9 +/- 0.6 years, teen-females 14.4 +/- 0.7 years, women 25.2 +/- 1.4) participated in this study. They performed high-intensity intermittent exercise (4 x 18 maximal knee flexions and extensions with 1-min rest) on an isokinetic dynamometer at 120 degrees s(-1). Peak torque of flexors (PTFL) and extensors (PTEX), and total work (TW) were measured. FR was calculated as % of PTEX, PTFL, and TW in 4th versus 1st set. FR was greater (P < 0.05) in boys versus teen-males and men, and in teen-males versus men. In females, FR was greater (P < 0.05) in girls versus teen-females and women, but not different between teen-females and women. FR was not different in boys versus girls and in teen-males versus teen-females. FR for PTFL, PTEX, and TW correlated negatively (P < 0.001) with the respective peak values (r = -0.68 to -0.84), and FR for TW with peak lactate (r = -0.58 to -0.69). In addition, age correlated (P < 0.01) with FR for males (r = -0.75) and females (r = -0.55). In conclusion, FR during high-intensity intermittent exercise undergoes a gradual decline from childhood to adulthood in males, while in females the adult profile establishes at mid-puberty (14-15 years). The maturation profile of FR in males and females during development appears to reflect the maturation profiles of peak torque, short-term muscle power, and lactate concentration after exercise.