Liang Xin-Mei, Tang Guang-Yu, Cheng Ying-Sheng, Zhou Bi
Department of Radiology, Sixth People's Hospital of Shanghai Jiaotong University; Institute of Medical Imaging of Shanghai Jiaotong University, No. 600, Yishan Road, Shanghai 200233, China.
World J Gastroenterol. 2009 May 7;15(17):2139-44. doi: 10.3748/wjg.15.2139.
To establish a rabbit rectal VX2 carcinoma model for the study of rectal carcinoma.
A suspension of VX2 cells was injected into the rectum wall under the guidance of X-ray fluoroscopy. Computed tomography (CT) and magnetic resonance imaging (MRI) were used to observe tumor growth and metastasis at different phases. Pathological changes and spontaneous survival time of the rabbits were recorded.
Two weeks after VX2 cell implantation, the tumor diameter ranged 4.1-5.8 mm and the success implantation rate was 81.8%. CT scanning showed low-density foci of the tumor in the rectum wall, while enhanced CT scanning demonstrated asymmetrical intensification in tumor foci. MRI scanning showed a low signal of the tumor on T(1)-weighted imaging and a high signal of the tumor on T(2)-weighted imaging. Both types of signals were intensified with enhanced MRI. Metastases to the liver and lung could be observed 6 wk after VX2 cell implantation, and a large area of necrosis appeared in the primary tumor. The spontaneous survival time of rabbits with cachexia and multiple organ failure was about 7 wk after VX2 cell implantation.
The rabbit rectal VX2 carcinoma model we established has a high stability, and can be used in the study of rectal carcinoma.
建立兔直肠VX2癌模型用于直肠癌研究。
在X线透视引导下将VX2细胞悬液注入直肠壁。采用计算机断层扫描（CT）和磁共振成像（MRI）观察不同阶段肿瘤的生长及转移情况。记录兔的病理变化及自然生存时间。
VX2细胞植入后2周，肿瘤直径为4.1 - 5.8 mm，植入成功率为81.8%。CT扫描显示直肠壁内肿瘤呈低密度灶，增强CT扫描显示肿瘤灶呈不均匀强化。MRI扫描显示肿瘤在T1加权像上呈低信号，在T2加权像上呈高信号。两种信号在增强MRI上均强化。VX2细胞植入后6周可观察到肝和肺转移，原发肿瘤出现大片坏死。恶病质和多器官功能衰竭兔的自然生存时间在VX2细胞植入后约7周。
我们建立的兔直肠VX2癌模型稳定性高，可用于直肠癌研究。
