Sun Jinglu, Song Xiaobo, Kristiansen Bjørn Erik, Kjaereng Anne, Willems Rob J L, Eriksen Harald M, Sundsfjord Arnfinn, Sollid Johanna E
Department of Medical Biology, Medical Faculty, University of Tromsø, Tromsø, Norway.
J Clin Microbiol. 2009 Jul;47(7):2218-25. doi: 10.1128/JCM.00388-09. Epub 2009 May 6.
Subgingival plaque samples and root canal samples were collected from 2,839 marginal periodontitis (MP) patients and 21 apical periodontitis (AP) patients. Enterococcus species were identified by a series of phenotypic and genotypic tests. Antimicrobial susceptibility assays were performed by an agar disk diffusion test. Multilocus sequence typing (MLST), eBURST, and minimum spanning tree were used for enterococcal genetic clustering and population analysis. Enterococcus faecalis was recovered from 3.7% MP patients and 9.5% AP patients, and Enterococcus faecium was recovered from 0.04% MP patients. Enterococci were detected more often in older male patients. E. faecalis isolates of MP were found resistant to tetracycline (49.1%), erythromycin (8.5%), trimethoprim (2.8%), and gentamicin (1.9%), while one AP isolate was resistant to tetracycline. A total of 40 sequence types (STs) were resolved in 108 E. faecalis isolates. Comparison with E. faecalis international MLST database revealed that 27 STs were previously found, 13 STs were novel, and several major clonal complexes in the database were also found in MP isolates. The tetracycline-resistant isolates distributed mainly in the major clonal complexes and singletons, whereas the erythromycin-resistant isolates were more dispersed. Although the rate of occurrence of enterococci recovered in the MP and AP samples was low, 50% of these isolates are resistant to at least one antimicrobial agent, which is most often tetracycline. This implies that subgingival E. faecalis might represent a reservoir of resistance to tetracycline and erythromycin. The subgingival E. faecalis isolates show high genetic diversity but are grouped, in general, with the known isolates from the international database.
从2839例边缘性牙周炎（MP）患者和21例根尖周炎（AP）患者中采集龈下菌斑样本和根管样本。通过一系列表型和基因型测试鉴定肠球菌属。采用琼脂纸片扩散法进行药敏试验。多位点序列分型（MLST）、eBURST和最小生成树用于肠球菌的基因聚类和群体分析。粪肠球菌在3.7%的MP患者和9.5%的AP患者中检出，屎肠球菌在0.04%的MP患者中检出。肠球菌在老年男性患者中检出率更高。MP患者的粪肠球菌分离株对四环素（49.1%）、红霉素（8.5%）、甲氧苄啶（2.8%）和庆大霉素（1.9%）耐药，而1株AP分离株对四环素耐药。108株粪肠球菌分离株共鉴定出40种序列型（STs）。与粪肠球菌国际MLST数据库比较发现，27种STs先前已被发现，13种STs为新发现，数据库中的几个主要克隆复合体在MP分离株中也有发现。耐四环素分离株主要分布在主要克隆复合体和单克隆中，而耐红霉素分离株分布更分散。虽然在MP和AP样本中回收的肠球菌发生率较低，但这些分离株中有50%对至少一种抗菌药物耐药，最常见的是四环素。这意味着龈下粪肠球菌可能是四环素和红霉素耐药性的储存库。龈下粪肠球菌分离株显示出高度的遗传多样性，但总体上与国际数据库中已知的分离株归为一类。
