Cheong Won Jo, Yang Song Hee
Department of Chemistry, Inha University, Incheon, South Korea.
Methods Mol Biol. 2013;970:469-87. doi: 10.1007/978-1-62703-263-6_30.
A generalized preparation protocol for open tubular (OT) molecular imprinted polymer (MIP) silica capillary columns for chiral separations in capillary electrochromatography (CEC) is described. For -different chiral molecules the same general composition of the MIP reaction mixture can be used except for the choice of the specific template. This protocol is valid for a variety of templates including acidic, basic, and neutral molecules, enabling formation of rugged and porous thin MIP layers on the inner surface of the capillary resulting in columns with high chiral separation efficiencies if the template has a good chiral recognition susceptibility.
描述了一种用于毛细管电色谱（CEC）手性分离的开管（OT）分子印迹聚合物（MIP）硅胶毛细管柱的通用制备方案。对于不同的手性分子，除了特定模板的选择外，MIP反应混合物的一般组成可以相同。该方案适用于包括酸性、碱性和中性分子在内的各种模板，如果模板具有良好的手性识别敏感性，则能够在毛细管内表面形成坚固且多孔的薄MIP层，从而得到具有高手性分离效率的柱子。
