Del Vecchio Giovanna, Giuliani Alessandro, Fernandez Mercedes, Mesirca Pietro, Bersani Ferdinando, Pinto Rosanna, Ardoino Lucia, Lovisolo Giorgio A, Giardino Luciana, Calzà Laura
BioPharmaNet-DIMORFIPA, Bologna University, Bologna, Italy.
Neurosci Lett. 2009 May 22;455(3):173-7. doi: 10.1016/j.neulet.2009.03.061. Epub 2009 Mar 24.
The effects of radiofrequency electromagnetic field (RF-EMF) exposure on neuronal phenotype maturation have been studied in two different in vitro models: murine SN56 cholinergic cell line and rat primary cortical neurons. The samples were exposed at a dose of 1W/kg at 900 MHz GSM modulated. The phenotype analysis was carried out at 48 and 72 h (24 and 48 h of SN56 cell line differentiation) or at 24, 72, 120 h (2, 4 and 6 days in vitro for cortical neurons) of exposure, on live and immunolabeled neurons, and included the morphological study of neurite emission, outgrowth and branching. Moreover, cortical neurons were studied to detect alterations in the expression pattern of cytoskeleton regulating factors, e.g. beta-thymosin, and of early genes, e.g. c-Fos and c-Jun through real-time PCR on mRNA extracted after 24h exposure to EMF. We found that RF-EMF exposure reduced the number of neurites generated by both cell systems, and this alteration correlates to increased expression of beta-thymosin mRNA.
在两种不同的体外模型中研究了射频电磁场（RF-EMF）暴露对神经元表型成熟的影响：小鼠SN56胆碱能细胞系和大鼠原代皮质神经元。样本以900 MHz GSM调制的1W/kg剂量进行暴露。在暴露48和72小时（SN56细胞系分化24和48小时）或24、72、120小时（皮质神经元体外培养2、4和6天）时，对活神经元和免疫标记的神经元进行表型分析，包括对神经突发出、生长和分支的形态学研究。此外，通过对暴露于电磁场24小时后提取的mRNA进行实时PCR，研究皮质神经元以检测细胞骨架调节因子（如β-胸腺素）和早期基因（如c-Fos和c-Jun）表达模式的变化。我们发现，RF-EMF暴露减少了两种细胞系统产生的神经突数量，并且这种改变与β-胸腺素mRNA表达增加相关。
