Han Yong, Gao Ling, Sun Nannan, Zhang Jie, Zhang Wei, Zhou Xinli, Zhang Haiqing, Zhao Jiajun
Department of Endocrinology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China.
J Ethnopharmacol. 2009 Apr 21;122(3):533-40. doi: 10.1016/j.jep.2009.01.023. Epub 2009 Feb 7.
Kang-Jia-Wan (KJW), a traditional Chinese herbal medicine, is widely used to treat goiter in the clinics in China.
The mechanisms by which KJW treats goiter are unclear. It is known that insufficient apoptosis of thyrocytes is involved in the formation of goiter. Here, we investigated whether KJW could induce apoptosis in goiter of rats given methimazole (MMI).
Fifty-six Wistar rats were randomly separated into four groups: normal, MMI, MMI+low-dose KJW and MMI+hig h-dose KJW. Except for the normal group (20 rats), all groups (each with 12 rats) were given 0.04% (w/v) MMI in their drinking water. One week later, the rats in MMI+low- and high-dose KJW groups were orally supplemented with KJW at 250 mg/kg d(-1) and 1000 mg/kg d(-1), respectively.
After KJW treatment for 12 weeks, the relative thyroid weight (mg/100g body weight) of the MMI+high-dose KJW group decreased significantly. Features of apoptosis were also apparent in thyroid tissues of rats given KJW treatment. Importantly, KJW markedly increased the caspase-3 and Fas protein expression, in a dose-dependent manner, in the thyroid specimens.
These results showed that KJW played a therapeutic role via apoptosis induction in the goitrous glands.
抗甲丸（KJW）是一种传统中药，在中国临床中广泛用于治疗甲状腺肿。
KJW治疗甲状腺肿的机制尚不清楚。已知甲状腺细胞凋亡不足与甲状腺肿的形成有关。在此，我们研究了KJW是否能诱导给予甲巯咪唑（MMI）的大鼠甲状腺肿发生细胞凋亡。
56只Wistar大鼠随机分为四组：正常组、MMI组、MMI+低剂量KJW组和MMI+高剂量KJW组。除正常组（20只大鼠）外，所有组（每组12只大鼠）饮用水中均给予0.04%（w/v）MMI。一周后，MMI+低剂量和高剂量KJW组大鼠分别以250mg/kg d（-1）和1000mg/kg d（-1）的剂量口服补充KJW。
KJW治疗12周后，MMI+高剂量KJW组的相对甲状腺重量（mg/100g体重）显著降低。给予KJW治疗的大鼠甲状腺组织中也出现了细胞凋亡特征。重要的是，KJW以剂量依赖的方式显著增加了甲状腺标本中caspase-3和Fas蛋白的表达。
这些结果表明，KJW通过诱导甲状腺肿腺细胞凋亡发挥治疗作用。
