Rao Gao-Xiong, Zhang Sen, Wang Hui-Min, Li Zhi-Min, Gao Suo, Xu Gui-Li
Department of Pharmacy, Kunming General Hospital of Chengdu Military Region, 212 Da-Guan Road, Kunming 650032, PR China.
J Ethnopharmacol. 2009 May 4;123(1):1-5. doi: 10.1016/j.jep.2009.02.046. Epub 2009 Mar 9.
The rattan stem of Fibraurea recisa Pierre. is known as an ethno-remedy commonly used for the treatment of various skin diseases by the minority Yao, Zhuang and Miao in Yunnan Province of China. The present study was designated to evaluate its antifungal activity, and to root out the antifungal substances from this ethical herb.
The in vitro antifungal assay was performed by agar diffusion test for extracts and fractions. Then, the active fractions were submitted to column chromatography on silica gel and LH-20 to isolate their compounds. And the antifungal activity of pure compounds has been examined by checkerboard microdilution test. Nine Candida strains and one Cryptococcus strain were used for the bioassay.
The MeOH extract exhibited significant antifungal activity, and the alkaloidal fractions were deduced as main active component. Subsequent studies led to the identification of a new alkaloid fibrecisine (1) and 21 known alkaloids including berberines, tetrahydroberberines and aporphine derivatives. The bioassay result indicated that the berberines showed more potent activity than aporphine derivatives against the test Candida strains, while tetrahydroberberines showed very weak activity against Cryptococcus neoformans.
The new alkaloid fibrecisine (1) was identified as 1,2-methylenedioxy-8-hydroxy-6a(R)-aporphine by detailed spectral analysis. The rattan stem of Fibraurea recisa Pierre. is an effective antifungal herb, and its major active component is alkaloidal compounds. Bioassay tests revealed that the water-soluble berberines are the most important antifungal substances. The study provides preliminary scientific validation for the traditional medicinal use of this ethno-remedy.
中国云南省的瑶族、壮族和苗族等少数民族常用黄藤茎作为民族药物治疗各种皮肤病。本研究旨在评估其抗真菌活性，并从这种民族草药中找出抗真菌物质。
采用琼脂扩散试验对提取物和馏分进行体外抗真菌试验。然后，将活性馏分进行硅胶柱色谱和LH-20柱色谱分离其化合物。并通过棋盘微量稀释试验检测纯化合物的抗真菌活性。使用9株念珠菌菌株和1株隐球菌菌株进行生物测定。
甲醇提取物表现出显著的抗真菌活性，生物碱馏分被推断为主要活性成分。后续研究鉴定出一种新生物碱黄藤素（1）和21种已知生物碱，包括小檗碱、四氢小檗碱和阿朴啡衍生物。生物测定结果表明，小檗碱对受试念珠菌菌株的活性比阿朴啡衍生物更强，而四氢小檗碱对新型隐球菌的活性非常弱。
通过详细的光谱分析，新生物碱黄藤素（1）被鉴定为1,2-亚甲二氧基-8-羟基-6a（R）-阿朴啡。黄藤茎是一种有效的抗真菌草药，其主要活性成分是生物碱类化合物。生物测定试验表明，水溶性小檗碱是最重要的抗真菌物质。该研究为这种民族药物的传统药用提供了初步的科学验证。
