Lau T W, Lam F F Y, Lau K M, Chan Y W, Lee K M, Sahota D S, Ho Y Y, Fung K P, Leung P C, Lau C B S
Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong.
J Ethnopharmacol. 2009 May 4;123(1):155-62. doi: 10.1016/j.jep.2009.02.010. Epub 2009 Feb 14.
Radix Rehmanniae (RR) has a very long history of usage in traditional Chinese medicine and is usually one of the principal herb found in many herbal formulae used in diabetic foot ulcer.
RR aqueous extract was investigated for its wound healing effects in a diabetic foot ulcer rat model and its detailed mechanism of actions.
A previously established diabetic foot ulcer rat model was used to assess the effect of RR extract on wound area reduction, tissue regeneration and angiogenesis. Carrageenan-induced inflammation rat model was used for inflammation study; and diabetic control was evaluated using a neonatal streptozotocin-induced diabetic rat model.
In the RR treated group, a trend of reduction of the wound area was observed from days 8 to 18 and a significant difference (as compared with control group) was found on day 8. The ulcer healing effect of RR extract was further supported by better developed scars and epithelialization as well as good formation of capillaries with enhanced VEGF expression. Carrageenan-induced inflammation was also significantly alleviated with RR extract.
Our results demonstrated for the first time that Radix Rehmanniae was effective in promoting diabetic foot ulcer healing in rats through the processes of tissue regeneration, angiogenesis and inflammation control, but not glycemia control. The present study provided scientific basis to support the traditional use of Radix Rehmanniae in diabetic foot ulcer.
地黄在传统中药中有着悠久的使用历史，并且通常是许多用于治疗糖尿病足溃疡的中药配方中的主要草药之一。
研究地黄水提取物对糖尿病足溃疡大鼠模型的伤口愈合作用及其详细作用机制。
使用先前建立的糖尿病足溃疡大鼠模型评估地黄提取物对伤口面积缩小、组织再生和血管生成的影响。使用角叉菜胶诱导的炎症大鼠模型进行炎症研究；并使用新生链脲佐菌素诱导的糖尿病大鼠模型评估糖尿病对照。
在地黄治疗组中，从第8天到第18天观察到伤口面积有减小趋势，并且在第8天发现与对照组有显著差异。地黄提取物的溃疡愈合效果还得到了更好的瘢痕形成和上皮化以及毛细血管良好形成和VEGF表达增强的进一步支持。地黄提取物对角叉菜胶诱导的炎症也有显著缓解作用。
我们的结果首次证明地黄通过组织再生、血管生成和炎症控制过程有效促进大鼠糖尿病足溃疡愈合，但对血糖控制无效。本研究为支持地黄在糖尿病足溃疡中的传统应用提供了科学依据。
