Conroy Paul J, Hearty Stephen, Leonard Paul, O'Kennedy Richard J
Biomedical Diagnostics Institute, National Centre for Sensor Research, School of Biotechnology, Dublin City University, Dublin 9, Ireland.
Semin Cell Dev Biol. 2009 Feb;20(1):10-26. doi: 10.1016/j.semcdb.2009.01.010. Epub 2009 Feb 4.
Currently, the reliable detection and quantification of a multitude of different analytes is crucial in many applications and settings. Biosensors have revolutionised diagnostics for use in point-of-care testing (POC), the detection of food and environmental contaminants, biological warfare agents, illicit drugs and human/animal disease markers. Antibodies continue to play a pivotal role in many sensor devices due to their exquisite specificity for their cognate antigens. In this review current biosensor platforms employing antibodies for molecular recognition are briefly described. The use of molecular biological techniques for the generation and improvement of antibodies is critically examined. Such recombinant antibodies possess improved attributes for use in biosensor development in terms of design, stability, affinity and specificity.
目前，在许多应用和场景中，可靠地检测和定量多种不同的分析物至关重要。生物传感器已经彻底改变了诊断方法，可用于即时检测（POC）、食品和环境污染物检测、生物战剂检测、非法药物检测以及人类/动物疾病标志物检测。由于抗体对其同源抗原具有极高的特异性，因此在许多传感设备中继续发挥着关键作用。在这篇综述中，简要描述了当前使用抗体进行分子识别的生物传感器平台。对用于抗体生成和改进的分子生物学技术进行了严格审查。此类重组抗体在设计、稳定性、亲和力和特异性方面具有更适合用于生物传感器开发的特性。
