Terentyuk Georgy S, Maslyakova Galina N, Suleymanova Leyla V, Khlebtsov Boris N, Kogan Boris Ya, Akchurin Garif G, Shantrocha Alexander V, Maksimova Irina L, Khlebtsov Nicolai G, Tuchin Valery V
Saratov State University, 83 Astrakhanskaya, 410012 Saratov, Russia.
J Biophotonics. 2009 May;2(5):292-302. doi: 10.1002/jbio.200910005.
Kinetics, biodistribution, and histological studies were performed to evaluate the particle-size effects on the distribution of 15 nm and 50 nm PEG-coated colloidal gold (CG) particles and 160 nm silica/gold nanoshells (NSs) in rats and rabbits. The above nanoparticles (NPs) were used as a model because of their importance for current biomedical applications such as photothermal therapy, optical coherence tomography, and resonance-scattering imaging. The dynamics of NPs circulation in vivo was evaluated after intravenous administration of 15 nm CG NPs to rabbit, and the maximal concentrations of gold were observed 15-30 min after injection. Rats were injected in the tail vein with PEG-coated NPs (about 0.3 mg Au/kg rats). 24 h after injection, the accumulation of gold in different organs and blood was determined by atomic absorption spectroscopy. In accordance with the published reports, we observed 15 nm particles in all organs with rather smooth distribution over liver, spleen and blood. By contrast, the larger NSs were accumulated mainly in the liver and spleen. For rabbits, the biodistribution was similar (72 h after intravenous injection). We report also preliminary data on the light microscopy and TEM histological examination that allows evaluation of the changes in biotissues after gold NPs treatment.
进行了动力学、生物分布和组织学研究，以评估粒径对15纳米和50纳米聚乙二醇包被的胶体金（CG）颗粒以及160纳米二氧化硅/金纳米壳（NSs）在大鼠和兔子体内分布的影响。上述纳米颗粒（NPs）被用作模型，因为它们对当前生物医学应用（如光热疗法、光学相干断层扫描和共振散射成像）具有重要意义。在给兔子静脉注射15纳米CG NPs后，评估了NPs在体内循环的动力学，注射后15 - 30分钟观察到金的最大浓度。给大鼠尾静脉注射聚乙二醇包被的NPs（约0.3毫克金/千克大鼠）。注射后24小时，通过原子吸收光谱法测定不同器官和血液中的金积累量。根据已发表的报告，我们在所有器官中都观察到了15纳米的颗粒，它们在肝脏、脾脏和血液中的分布相当均匀。相比之下，较大的NSs主要积聚在肝脏和脾脏中。对于兔子，生物分布情况相似（静脉注射后72小时）。我们还报告了关于光学显微镜和透射电子显微镜组织学检查的初步数据，这些数据有助于评估金纳米颗粒处理后生物组织的变化。
