Nakamura S, Morita T, Iwanaga S, Niwa M, Takahashi K
J Biochem. 1977 May;81(5):1567-9.
An endotoxin-activated hemocyte lysate from horseshoe crab (Tachypleus and Limulus) was found to hydrolyze specifically BZ-Ile-Glu-Gly-Arg-p-nitroanilide, which was recently introduced as the substrate for assay of the blood coagulation factor, Factor Xa. Further, this amidase activity increased by increasing the concentration of bacterial endotoxin (Salmonella minnesota R595) added to the lysate. Thus, the measurement of the amidase activity in the hemocyte lysate can be very useful to detect and determine the endotoxin.
人们发现，来自鲎（东方鲎和美洲鲎）的内毒素激活血细胞裂解物能特异性水解BZ-Ile-Glu-Gly-Arg-对硝基苯胺，该物质最近被用作检测凝血因子Xa的底物。此外，通过增加添加到裂解物中的细菌内毒素（明尼苏达沙门氏菌R595）的浓度，这种酰胺酶活性会增强。因此，测量血细胞裂解物中的酰胺酶活性对于检测和测定内毒素可能非常有用。
