骨骼肌对18F标记的6-氟-6-脱氧-D-葡萄糖的摄取对胰岛素刺激有反应。
Uptake of 18F-labeled 6-fluoro-6-deoxy-D-glucose by skeletal muscle is responsive to insulin stimulation.
作者信息
Spring-Robinson Chandra, Chandramouli Visvanathan, Schumann William C, Faulhaber Peter F, Wang Yanming, Wu Chunying, Ismail-Beigi Faramarz, Muzic Raymond F
机构信息
Department of Biomedical Engineering, University Hospitals Case Medical Center, School of Medicine, Case Western Reserve University, Cleveland, Ohio, USA.
出版信息
J Nucl Med. 2009 Jun;50(6):912-9. doi: 10.2967/jnumed.109.062687. Epub 2009 May 14.
UNLABELLED
We are developing a methodology for the noninvasive imaging of glucose transport in vivo with PET and (18)F-labeled 6-fluoro-6-deoxy-d-glucose ((18)F-6FDG), a tracer that is transported but not phosphorylated. To validate the method, we evaluated the biodistribution of (18)F-6FDG to test whether it is consistent with the known properties of glucose transport, particularly with regard to insulin stimulation of glucose transport.
METHODS
Under glucose clamp conditions, rats were imaged at the baseline and under conditions of hyperinsulinemia.
RESULTS
The images showed that the radioactivity concentration in skeletal muscle was higher in the presence of insulin than at the baseline. We also found evidence that the metabolism of (18)F-6FDG was negligible in several tissues.
CONCLUSION
(18)F-6FDG is a valid tracer that can be used in in vivo transport studies. PET studies performed under glucose clamp conditions demonstrated that the uptake of nonphosphorylated glucose transport tracer (18)F-6FDG is sensitive to insulin stimulation.
未标注
我们正在开发一种利用正电子发射断层扫描（PET）和（18）F标记的6-氟-6-脱氧-D-葡萄糖（（18）F-6FDG）对体内葡萄糖转运进行无创成像的方法，（18）F-6FDG是一种被转运但不被磷酸化的示踪剂。为验证该方法，我们评估了（18）F-6FDG的生物分布，以测试其是否与已知的葡萄糖转运特性一致，特别是在胰岛素刺激葡萄糖转运方面。
方法
在葡萄糖钳夹条件下，对大鼠在基线状态和高胰岛素血症状态下进行成像。
结果
图像显示，存在胰岛素时骨骼肌中的放射性浓度高于基线水平。我们还发现有证据表明（18）F-6FDG在几个组织中的代谢可忽略不计。
结论
（18）F-6FDG是一种可用于体内转运研究的有效示踪剂。在葡萄糖钳夹条件下进行的PET研究表明，未磷酸化的葡萄糖转运输 tracer（18）F-6FDG的摄取对胰岛素刺激敏感。
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