Institute of Biochemistry and Molecular Biology, School of Medicine, Shandong University, Shandong, People's Republic of China.
Mol Biol Rep. 2010 Mar;37(3):1619-25. doi: 10.1007/s11033-009-9574-7. Epub 2009 May 15.
Neuromyelitis optica (NMO) is an inflammatory demyelinating disease with generally poor prognosis that selectively targets optic nerves and spinal cord. Although diagnostic criteria for NMO are available, there is still a need for biomarkers, predicting disease development and progression to improve individually tailored treatment. CSF proteins were separated by two-dimensional electrophoresis and identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). The interaction between these proteins was further analyzed by Pathway Studio software. Seven protein spots in CSF were significantly altered in NMO patients compared with controls. Identification made by mass spectrometry revealed that the most significant protein was haptoglobin, which was increased in the NMO gels. The subsequent ELISA test were performed to validate it, which confirmed the results of proteomic analysis. Protein network was built, which showed some biological interactions among the seven proteins. These results support a correlation between the level of haptoglobin and NMO. Haptoglobin may be a potential useful biomarker for diagnosis or a medicine target for treatment of NMO.
视神经脊髓炎（NMO）是一种炎症性脱髓鞘疾病，预后一般较差，选择性地针对视神经和脊髓。虽然有 NMO 的诊断标准，但仍需要生物标志物来预测疾病的发展和进展，以改善个体化治疗。CSF 蛋白通过二维电泳分离，并通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）鉴定。通过 Pathway Studio 软件进一步分析这些蛋白质之间的相互作用。与对照组相比，NMO 患者的 CSF 中有 7 个蛋白点明显改变。通过质谱鉴定发现，最显著的蛋白是触珠蛋白，它在 NMO 凝胶中增加。随后进行 ELISA 检测以验证，这证实了蛋白质组学分析的结果。建立了蛋白质网络，显示了这 7 种蛋白质之间的一些生物学相互作用。这些结果支持触珠蛋白水平与 NMO 之间的相关性。触珠蛋白可能是 NMO 诊断的潜在有用生物标志物或治疗药物靶点。
