视神经脊髓炎中的脑脊液轻神经丝和重神经丝。
Cerebrospinal fluid light and heavy neurofilaments in neuromyelitis optica.
机构信息
Department of Neurology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui Province, China; Multiple Sclerosis Center, Department of Neurology, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong Province, China.
出版信息
Neurochem Int. 2013 Dec;63(8):805-8. doi: 10.1016/j.neuint.2013.10.008. Epub 2013 Oct 23.
BACKGROUND
Axonal injury is the correlate of disease progression in NMO and MS. Neurofilament (Nf) belongs to neuron specific intermediate filaments located in axons. Nf protein subunits are potential biomarkers in cerebrospinal fluid (CSF) for acute axonal injury. However, whether CSF NfH and NfL levels are elevated in NMO patients has remained unclear.
METHODS
Nf light subunit (NfL) and Nf heavy subunit NfH in cerebrospinal fluid were measured by enzyme-linked immunosorbent assay (ELISA) in NMO (n=32), MS (n=25), and other non-inflammatory neurological disease patients (OND, n=18).
RESULTS
CSF pNf-H levels were increased in the NMO patients compared with OND patients (p=0.001). CSF NfL levels in the NMO patients were also higher compared with MS patients (p=0.001), and OND patients (p=0.000001). When comparing NfL levels between MS and OND patients, there also significant differences (p=0.0003). NMO and MS patients revealed a trend to an increased disability with increased CSF NfL during relapse (NMO: p=0.006; MS: p=0.017). There is positive relationship between CSF pNf-H and disability of MS patients (p=0.041).
CONCLUSIONS
CSF levels of NfL are increased in NMO patients and reflect the disease severity in NMO.
背景
轴突损伤是 NMO 和 MS 疾病进展的相关因素。神经丝（Nf）属于位于轴突中的神经元特异性中间丝。神经丝蛋白亚基是脑脊液（CSF）中急性轴突损伤的潜在生物标志物。然而，NMO 患者的 CSF NfH 和 NfL 水平是否升高仍不清楚。
方法
通过酶联免疫吸附试验（ELISA）测量 NMO（n=32）、MS（n=25）和其他非炎症性神经疾病患者（OND，n=18）的 CSF 轻链（NfL）和 Nf 重链 NfH。
结果
与 OND 患者相比，NMO 患者的 CSF pNf-H 水平升高（p=0.001）。与 MS 患者相比，NMO 患者的 CSF NfL 水平也更高（p=0.001），与 OND 患者相比（p=0.000001）。当比较 MS 和 OND 患者之间的 NfL 水平时，也有显著差异（p=0.0003）。在复发期间，NMO 和 MS 患者的 CSF NfL 水平升高与残疾增加呈趋势（NMO：p=0.006；MS：p=0.017）。MS 患者的 CSF pNf-H 与残疾呈正相关（p=0.041）。
结论
NMO 患者的 CSF NfL 水平升高，反映了 NMO 的疾病严重程度。
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