Basso Katia, Sumazin Pavel, Morozov Pavel, Schneider Christof, Maute Roy L, Kitagawa Yukiko, Mandelbaum Jonathan, Haddad Joseph, Chen Chang-Zheng, Califano Andrea, Dalla-Favera Riccardo
Institute of Cancer Genetics and Herbert Irving Comprehensive Cancer Center, Columbia University, New York, NY 10032, USA.
Immunity. 2009 May;30(5):744-52. doi: 10.1016/j.immuni.2009.03.017. Epub 2009 May 14.
The full set of microRNAs (miRNAs) in the human genome is not known. Because presently known miRNAs have been identified by virtue of their abundant expression in a few cell types, many tissue-specific miRNAs remain unrevealed. To understand the role of miRNAs in B cell function and lymphomagenesis, we generated short-RNA libraries from normal human B cells at different stages of development (naive, germinal center, memory) and from a Burkitt lymphoma cell line. A combination of cloning and computational analysis identified 178 miRNAs (miRNome) expressed in normal and/or transformed B cell libraries. Most notably, the B cell miRNome included 75 miRNAs which to our knowledge have not been previously reported and of which 66 have been validated by RNA blot and/or RT-PCR analyses. Numerous miRNAs were expressed in a stage- or transformation-specific fashion in B cells, suggesting specific functional or pathologic roles. These results provide a resource for studying the role of miRNAs in B cell development, immune function, and lymphomagenesis.
人类基因组中微小RNA（miRNA）的完整集合尚不清楚。由于目前已知的miRNA是通过它们在少数细胞类型中的高丰度表达而被鉴定出来的，许多组织特异性miRNA仍未被发现。为了了解miRNA在B细胞功能和淋巴瘤发生中的作用，我们从处于不同发育阶段（初始、生发中心、记忆）的正常人B细胞以及一个伯基特淋巴瘤细胞系中生成了短RNA文库。克隆和计算分析相结合，鉴定出在正常和/或转化的B细胞文库中表达的178种miRNA（miRNA组）。最值得注意的是，B细胞miRNA组包含75种miRNA，据我们所知，此前尚未有相关报道，其中66种已通过RNA印迹和/或逆转录-聚合酶链反应分析得到验证。许多miRNA在B细胞中以阶段特异性或转化特异性的方式表达，提示其具有特定的功能或病理作用。这些结果为研究miRNA在B细胞发育、免疫功能和淋巴瘤发生中的作用提供了资源。
