富血小板纤维蛋白（PRF）中生长因子的体外释放：优化PRF临床应用的建议
In vitro release of growth factors from platelet-rich fibrin (PRF): a proposal to optimize the clinical applications of PRF.
作者信息
Su Chen Yao, Kuo Ya Po, Tseng Yu Hong, Su Ching-Hua, Burnouf Thierry
机构信息
Department of Dentistry, National Yang-Ming University, Taipei, Taiwan.
出版信息
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009 Jul;108(1):56-61. doi: 10.1016/j.tripleo.2009.02.004. Epub 2009 May 17.
OBJECTIVE
Determine the release of growth factors (GF) from platelet-rich fibrin (PRF) and supernatant serum to optimize clinical use.
STUDY DESIGN
Platelet-derived growth factors-AB (PDGF-AB), transforming growth factor-beta1 (TGF-beta1), vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), and insulin-like growth factor-1 (IGF-1) were quantified in PRF releasate and in the supernatant serum (N = 8) over 300 minutes after clot formation. Protein profiles were determined by SDS-PAGE.
RESULTS
Mean quantity of PDGF-AB, TGF-ss1, VEGF, and EGF in PRF releasate increased significantly to about 52, 72, 1, and 3 ng, respectively, whereas mean IGF-1 content remained at 250 ng. GF was also found in serum supernatant. Protein profiles of the releasates and the supernatant serum were similar.
CONCLUSION
The PRF membrane should be used immediately after formation to maximize release of GF to the surgical site. The remaining fluid can be recovered as an additional source of GF for grafting.
目的
确定富血小板纤维蛋白（PRF）和上清血清中生长因子（GF）的释放情况，以优化临床应用。
研究设计
在血凝块形成后的300分钟内，对富血小板纤维蛋白释放物和上清血清（N = 8）中的血小板衍生生长因子-AB（PDGF-AB）、转化生长因子-β1（TGF-β1）、血管内皮生长因子（VEGF）、表皮生长因子（EGF）和胰岛素样生长因子-1（IGF-1）进行定量分析。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）测定蛋白质谱。
结果
富血小板纤维蛋白释放物中PDGF-AB、TGF-β1、VEGF和EGF的平均含量分别显著增加至约52、72、1和3 ng，而IGF-1的平均含量保持在250 ng。在血清上清液中也发现了生长因子。释放物和上清血清的蛋白质谱相似。
结论
富血小板纤维蛋白膜形成后应立即使用，以使生长因子向手术部位的释放最大化。剩余液体可作为用于移植的生长因子的额外来源回收。
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