通过印迹杂交揭示的人类Alu亚家族及其甲基化

Department of Genetics, University of California, Davis 95616.

Nucleic Acids Res. 1991 Oct 25;19(20):5613-7. doi: 10.1093/nar/19.20.5613.

By a simple direct blot hybridization strategy, the existence of human Alu family subfamilies is confirmed. Using consensus restriction cleavage sites, individual bands can be resolved from genomic human DNA digests corresponding to three distinct Alu subfamilies. Digestion with methylation sensitive and insensitive restriction enzymes shows that the numerous CpG residues in the youngest Alu subfamilies are largely methylated in vivo, suggesting a model for the transcriptional regulation of Alu repeats.

通过一种简单的直接印迹杂交策略，证实了人类Alu家族亚家族的存在。利用共有限制性酶切位点，可以从对应于三个不同Alu亚家族的人类基因组DNA消化产物中分辨出各个条带。用对甲基化敏感和不敏感的限制性酶进行消化表明，最年轻的Alu亚家族中的大量CpG残基在体内大部分被甲基化，这提示了一种Alu重复序列转录调控的模型。