Małek Wanda, Wdowiak-Wróbel Sylwia, Bartosik Michał, Konopa Grazyna, Narajczyk Magdalena
Department of Genetics and Microbiology, M. Curie-Skłodowska University, Akademicka 19 Street, Lublin, 20-033, Poland.
Curr Microbiol. 2009 Aug;59(2):187-92. doi: 10.1007/s00284-009-9421-z. Epub 2009 May 21.
Three lytic phages (PhiRP1, PhiRP2, and PhiRP3) specific for Robinia pseudoacacia rhizobia were isolated from the soil under black locust. They were characterized by their morphology, host range, and some other properties including DNA molecular weights. Studied phages have been found to belong to Siphoviridae family that comprises viruses with long, and noncontractile tails. They had broad host ranges and effectively lysed not only Robinia pseudoacacia microsymbionts but also different Mesorhizobium species. The phages were homogenous in latent periods (300 min) but heterogeneous in burst sizes (100-200 phage particles per one infected cell) and rise periods (90-120 min). They showed a distinct adsorption rate to Robinia pseudoacacia rhizobia (70.4-93.94%). The molecular weights of phage DNAs estimated from restriction enzyme digests were in the range from ca. 82 kb to ca. 105 kb.
从刺槐林下土壤中分离出三种对刺槐根瘤菌具有特异性的裂解性噬菌体（PhiRP1、PhiRP2和PhiRP3）。对它们进行了形态学、宿主范围以及包括DNA分子量在内的其他一些特性的表征。研究发现这些噬菌体属于长尾噬菌体科，该科病毒具有长且不可收缩的尾部。它们具有广泛的宿主范围，不仅能有效裂解刺槐微共生体，还能裂解不同的中生根瘤菌物种。这些噬菌体的潜伏期相同（300分钟），但爆发量（每个被感染细胞产生100 - 200个噬菌体颗粒）和上升期（90 - 120分钟）存在差异。它们对刺槐根瘤菌表现出明显的吸附率（70.4 - 93.94%）。通过限制性酶切分析估计的噬菌体DNA分子量在约82 kb至约105 kb范围内。
