对顶端和各向同性芽生长存在缺陷的酵母缺失突变体进行全面定量分析。
Comprehensive and quantitative analysis of yeast deletion mutants defective in apical and isotropic bud growth.
作者信息
Watanabe Machika, Watanabe Daisuke, Nogami Satoru, Morishita Shinichi, Ohya Yoshikazu
机构信息
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba, 277-8562, Japan.
出版信息
Curr Genet. 2009 Aug;55(4):365-80. doi: 10.1007/s00294-009-0251-0. Epub 2009 May 24.
To obtain a comprehensive understanding of the budding phase transition, 4,711 Saccharomyces cerevisiae haploid nonessential gene deletion mutants were screened with the image processing program CalMorph, and 35 mutants with a round bud and 173 mutants with an elongated bud were statistically identified. We classified round and elongated bud mutants based on factors thought to affect the duration of the apical bud growth phase. Two round bud mutants (arc18 and sac6) were found to be defective in apical actin patch localization. Several elongated bud mutants demonstrated a delay of cell cycle progression at the apical growth phase, suggesting that these mutants have a defect in the control of cell cycle progression.
为全面了解出芽期转变，使用图像处理程序CalMorph对4711个酿酒酵母单倍体非必需基因缺失突变体进行了筛选，经统计鉴定出35个具有圆形芽的突变体和173个具有伸长芽的突变体。我们根据认为会影响顶端芽生长阶段持续时间的因素，对圆形芽和伸长芽突变体进行了分类。发现两个圆形芽突变体（arc18和sac6）在顶端肌动蛋白斑定位方面存在缺陷。几个伸长芽突变体在顶端生长阶段表现出细胞周期进程延迟，这表明这些突变体在细胞周期进程控制方面存在缺陷。
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