School of Chemical Engineering and Analytical Science, Manchester Interdisciplinary Biocentre, The University of Manchester, 131 Princess Street, Manchester, UK M1 7DN.
Analyst. 2009 Jun;134(6):1171-5. doi: 10.1039/b821349f. Epub 2009 Apr 9.
Fourier transform infrared spectra of a single cell in transflection geometry are seen to vary significantly with position on the cell, showing a distorted derivative-like lineshape in the region of the optically dense nucleus. A similar behaviour is observable in a model system of the protein albumin doped in a potassium bromide disk. It is demonstrated that the spectrum at any point is a weighted sum of the sample reflection and transmission and that the dominance of the reflection spectrum in optically dense regions can account for some of the spectral distortions previously attributed to dispersion artefacts. Rather than being an artefact, the reflection contribution is ever present in transflection spectra and it is further demonstrated that the reflection characteristics can be used for cellular mapping.
在反射几何中，单个细胞的傅里叶变换红外光谱随细胞位置发生显著变化，在光密度核区域显示出扭曲的类似导数的线形状。在蛋白质白蛋白掺杂在溴化钾盘中的模型系统中可以观察到类似的行为。结果表明，任意点的光谱是样品反射和透射的加权和，并且在光密度区域中反射光谱的主导地位可以解释一些先前归因于色散伪像的光谱扭曲。在反射光谱中，反射贡献不仅是一个伪像，而且进一步证明了反射特性可用于细胞映射。
