Bräuer Lars, Möschter Susann, Beileke Stephanie, Jäger Kristin, Garreis Fabian, Paulsen Friedrich P
Department of Anatomy and Cell Biology, Martin Luther University of Halle-Wittenberg, Grosse Steinstr. 52, 06097, Halle/Saale, Germany.
Histochem Cell Biol. 2009 Sep;132(3):331-8. doi: 10.1007/s00418-009-0609-x. Epub 2009 May 30.
The oral cavity and the salivary glands are open to the oral environment and are thus exposed to multiple microbiological, chemical and mechanical influences. The existence of an efficient defense system is essential to ensure healthy and physiological function of the oral cavity. Surfactant proteins play an important role in innate immunity and surface stability of fluids. This study aimed to evaluate the expression and presence of surfactant proteins (SP) A, B, C, and D in human salivary glands and saliva. The expression of mRNA for SP-A, -B, -C and -D was analyzed by RT-PCR in healthy parotid and submandibular glands. Deposition of all surfactant proteins was determined with monoclonal antibodies by means of Western blot analysis and immunohistochemistry in healthy tissues and saliva of volunteers. Our results show that all four surfactant proteins SP-A, SP-B, SP-C and SP-D are peptides of saliva and salivary glands. Based on the known direct and indirect antimicrobial effects of collectins, the surfactant-associated proteins A and D appear to be involved in immune defense inside the oral cavity. Furthermore, by lowering surface tension between saliva and the epithelial lining of excretory ducts, SP-B and SP-C may assist in drainage and outflow into the oral cavity. Further functions such as pellicle formation on teeth have yet to be determined.
口腔和唾液腺与口腔环境相通，因此会受到多种微生物、化学和机械因素的影响。高效防御系统的存在对于确保口腔的健康和生理功能至关重要。表面活性蛋白在固有免疫和液体的表面稳定性中发挥着重要作用。本研究旨在评估表面活性蛋白（SP）A、B、C和D在人类唾液腺和唾液中的表达及存在情况。通过逆转录聚合酶链反应（RT-PCR）分析了健康腮腺和颌下腺中SP-A、-B、-C和-D的信使核糖核酸（mRNA）表达。借助蛋白质免疫印迹分析和免疫组织化学方法，用单克隆抗体测定了健康志愿者组织和唾液中所有表面活性蛋白的沉积情况。我们的结果表明，所有四种表面活性蛋白SP-A、SP-B、SP-C和SP-D都是唾液和唾液腺中的肽类物质。基于已知的凝集素的直接和间接抗菌作用，表面活性相关蛋白A和D似乎参与口腔内的免疫防御。此外，通过降低唾液与排泄管上皮衬里之间的表面张力，SP-B和SP-C可能有助于引流并流入口腔。诸如在牙齿上形成菌斑等其他功能还有待确定。
