次氯酸钠对体外人外周血淋巴细胞的细胞毒性和遗传毒性作用。
Cytotoxic and genotoxic effects of sodium hypochlorite on human peripheral lymphocytes in vitro.
机构信息
Department of Biology, Faculty of Sciences and Arts, University of Kafkas, Kars, Turkey,
出版信息
Cytotechnology. 2009 Mar;59(2):113-9. doi: 10.1007/s10616-009-9201-4. Epub 2009 May 30.
Abstract
Chlorination is widely used method in the disinfection of drinking and utility water worldwide. In this study, cytotoxic and genotoxic effects of sodium hypochlorite were investigated by the cytokinesis-block micronucleus assay and chromosomal aberration analysis on human peripheral lymphocytes in vitro. A significant increase in chromosomal aberration frequency was observed in all treatments of NaOCl (0.030, 0.065, 0.100, 0.25, 0.5, 1, 2, 4 mug/mL) at 24 and 48 h compared with the negative control and mitomycin C (MMC, 0.3 mug/mL), which was used as a positive control. NaOCl significantly increased the frequency of micronuclei in a dose dependent manner. The results showed that there was a significant correlation between NaOCl concentration and chromosomal aberration, micronuclei frequency, necrotic cells, apoptotic cells and binucleated cells.
摘要
氯化是全球饮用水和实用水消毒的广泛应用方法。在这项研究中，通过细胞分裂阻断微核试验和体外人外周血淋巴细胞染色体畸变分析，研究了次氯酸钠的细胞毒性和遗传毒性作用。与阴性对照和丝裂霉素 C（MMC，0.3μg/mL）相比，所有次氯酸钠处理组（0.030、0.065、0.100、0.25、0.5、1、2、4μg/mL）在 24 和 48 小时时，染色体畸变频率均显著增加，MMC 被用作阳性对照。次氯酸钠显著增加微核的频率呈剂量依赖性。结果表明，次氯酸钠浓度与染色体畸变、微核频率、坏死细胞、凋亡细胞和双核细胞之间存在显著相关性。
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