通过聚合酶链反应检测骆驼痘病毒和牛痘病毒。

Detection of camel pox and vaccinia viruses by polymerase chain reaction.

作者信息

Sheikh Ali Hanan M, Khalafalla A I, Nimir A H

机构信息

Department of Microbiology, College of Veterinary Medicine & Animal Production, Sudan University of Science & Technology, P.O. Box: 204, Khartoum North, Sudan.

出版信息

Trop Anim Health Prod. 2009 Dec;41(8):1637-41. doi: 10.1007/s11250-009-9359-y. Epub 2009 May 30.

DOI:10.1007/s11250-009-9359-y

PMID:19484375

Abstract

PCR following two methods of DNA extraction was used to confirm the growth of camel pox virus (CPV) and vaccinia virus in cell culture and chorioallantoic membrane. Results were compared with the commonly used neutralization test. The first method of DNA extraction was accomplished by using viral DNA in tissue culture supernatant and Chorioallantoic membrane, which was released by initial heating for 15 min at 99 degrees C followed by ordinary PCR. In the second method DNA was extracted by using DNA Isolation Kit from tissue culture supernatant and used as a template. Rapid identification and differentiation of CPV and Vaccinia virus were achieved by PCR and this assay proved to be fast and feasible, and can be an alternative to orthodox serological methods.

摘要

采用两种DNA提取方法后的聚合酶链反应（PCR），用于确认骆驼痘病毒（CPV）和痘苗病毒在细胞培养物和绒毛尿囊膜中的生长情况。将结果与常用的中和试验进行比较。第一种DNA提取方法是利用组织培养上清液和绒毛尿囊膜中的病毒DNA，先在99摄氏度下加热15分钟以释放病毒DNA，然后进行常规PCR。第二种方法是使用DNA分离试剂盒从组织培养上清液中提取DNA并用作模板。通过PCR实现了CPV和痘苗病毒的快速鉴定和区分，该检测方法被证明快速可行，可作为传统血清学方法的替代方法。