United States Department of Agriculture, Agricultural Research Service, Southern Plains Agricultural Research Center, Food and Feed Safety Research Unit, 2881 F&B Road, College Station, Texas, USA.
J Appl Microbiol. 2009 Oct;107(4):1258-68. doi: 10.1111/j.1365-2672.2009.04308.x. Epub 2009 Apr 17.
To determine if the purported deaminase inhibitors diphenyliodonium chloride (DIC) and thymol reduce the growth and survivability of Campylobacter.
Growth rates of Campylobacter jejuni and Camp. coli were reduced compared to unsupplemented controls during culture in Muellar-Hinton broth supplemented with 0.25 micromol DIC or thymol ml(-1) but not with 0.01 micromol monensin ml(-1) or 1% ethanol. Recovery of Camp. jejuni and Camp. coli was reduced >5 log(10) CFU from controls after 24 h pure culture in Bolton broth supplemented with 0.25 or 1.0 micromol DIC ml(-1) or with 1.0 micromol thymol ml(-1). Similarly, each test Campylobacter strain was reduced >3 log(10) CFU from controls after 24 h mixed culture with porcine faecal microbes in Bolton broth supplemented with 0.25 or 1.0 micromol DIC ml(-1) or with 1.0 micromol thymol ml(-1). Treatments with 0.25 micromol thymol ml(-1), 0.01 micromol monensin ml(-1) or 1% ethanol were less effective. Ammonia production during culture or incubation of cell lysates was reduced by 0.25 or 1.0 micromol DIC ml(-1) but only intermittently reduced, if at all, by the other treatments.
Diphenyliodonium chloride and thymol reduced growth, survivability and ammonia production of Camp. jejuni and Camp. coli.
Results suggest a potential physiological characteristic that may be exploited to develop interventions.
确定所谓的脱氨酶抑制剂二苯基碘氯化物（DIC）和百里酚是否能降低弯曲菌的生长和存活能力。
在补充有 0.25 微摩尔 DIC 或百里酚 ml(-1)的 Mueller-Hinton 肉汤中培养时，与未补充对照相比，空肠弯曲菌和结肠弯曲菌的生长速度降低，但补充有 0.01 微摩尔莫能菌素 ml(-1)或 1%乙醇时则不然。在补充有 0.25 或 1.0 微摩尔 DIC ml(-1)或 1.0 微摩尔百里酚 ml(-1)的 Bolton 肉汤中进行 24 小时纯培养后，从对照中回收空肠弯曲菌和结肠弯曲菌的数量减少了>5 log(10) CFU。同样，在补充有 0.25 或 1.0 微摩尔 DIC ml(-1)或 1.0 微摩尔百里酚 ml(-1)的 Bolton 肉汤中进行 24 小时混合培养后，每种试验弯曲菌菌株从对照中减少的数量都超过了>3 log(10) CFU。用 0.25 微摩尔百里酚 ml(-1)、0.01 微摩尔莫能菌素 ml(-1)或 1%乙醇处理的效果较差。在培养或细胞裂解物孵育过程中，氨的产生被 0.25 或 1.0 微摩尔 DIC ml(-1)减少，但只有在其他处理中被间歇性地减少，如果有的话。
二苯基碘氯化物和百里酚降低了空肠弯曲菌和结肠弯曲菌的生长、存活和氨产生。
结果表明，可能存在一种生理特征，可以利用它来开发干预措施。
