Departamento de Nutrición, Bromatología y Tecnología de Alimentos, Universidad Complutense de Madrid, Madrid, Spain.
Zoonoses Public Health. 2010 Sep;57(6):402-10. doi: 10.1111/j.1863-2378.2009.01232.x.
In an attempt to study the diversity and persistence of molecular subtypes of pathogenic Listeria spp. in a cheese factory at the La Mancha region of Spain, 43 samples were taken from incoming raw milk (cow's, ewe's, goat's and mixed species) and from certain food-contact and environmental surfaces before and/or after sanitation. Of these samples, 12 contained pathogenic Listeria. From the chromogenic agar plates corresponding to those, 46 phosphatidylinositol-specific phospholipase C-positive isolates were randomly taken for further analysis, including biochemical tests and pulsed-field gel electrophoresis (PFGE). They coincided in identifying all the 46 as Listeria ivanovii subsp. ivanovii, apparently a single PFGE type. Both ewe's and goat's raw milk batches from asymptomatic animals tested along the 6-month period persistently carried the same strain, which was also obtained from inner surfaces of raw milk truck tanks and the milk dump tank at the cheese factory. Biofilm-forming abilities of this L. ivanovii clone and interference against L. monocytogenes Scott A reference strain were tested, but failed to account for the clone's apparent pervasive presence.
为了研究西班牙拉曼恰地区一家奶酪工厂中病原性李斯特菌属分子亚型的多样性和持久性，从进厂的原料奶（奶牛、绵羊、山羊和混合物种）以及卫生前后的某些食品接触面和环境表面采集了 43 个样本。这些样本中有 12 个含有病原性李斯特菌。从相应的显色琼脂平板上随机抽取了 46 个磷脂酰肌醇特异性磷脂酶 C 阳性分离株进行进一步分析，包括生化试验和脉冲场凝胶电泳（PFGE）。它们都将 46 个分离株鉴定为李斯特菌 ivanovii 亚种 ivanovii，显然是单一的 PFGE 型。在 6 个月的时间里，来自无症状动物的绵羊和山羊原料奶批次持续携带相同的菌株，该菌株也从原料奶罐车的内部表面和奶酪厂的牛奶倾卸罐中获得。测试了该李斯特菌克隆的生物膜形成能力和对李斯特菌单核细胞增生性李斯特菌参考株的干扰，但未能解释该克隆的明显普遍存在。
