Ramage C P, Low J C, McLauchlin J, Donachie W
Moredun Research Institute, Pentland Science Park, Penicuik, Midlothian, UK.
FEMS Microbiol Lett. 1999 Jan 15;170(2):349-53. doi: 10.1111/j.1574-6968.1999.tb13394.x.
Forty-three Listeria ivanovii isolates were collected in the UK between 1991 and 1997 from: 35 animal infections; two human infections; five foods; and one environmental source. A further two type strains of L. ivanovii (subsp. ivanovii and subsp. londoniensis) were obtained from a culture collection. These bacteria were characterised by conventional phenotypic methods and by pulsed-field gel electrophoresis (PFGE) using ApaI and SmaI. Forty-two of the isolates from the UK were identified as L. ivanovii subsp. ivanovii and the remaining culture as L. ivanovii subsp. londoniensis. Six and four PFGE profiles were obtained using ApaI and SmaI digestion respectively; six composite profiles were obtained combining the results for both enzymes. The PFGE profile of the UK L. ivanovii subsp. londoniensis (isolated from processed shrimps) was similar to the type strain of this subspecies and differed from all of the L. ivanovii subsp. ivanovii tested. The majority of isolates (38 out of 45) belonged to one profile showing that the UK population of this bacterium is much less genetically diverse than similar studies have shown for Listeria monocytogenes.
1991年至1997年间，在英国收集了43株伊氏李斯特菌分离株，来源如下：35例动物感染；2例人类感染；5份食品；以及1个环境源。另外从菌种保藏中心获得了2株伊氏李斯特菌的标准菌株（伊氏亚种和伦敦亚种）。这些细菌通过传统表型方法以及使用ApaI和SmaI的脉冲场凝胶电泳（PFGE）进行鉴定。来自英国的42株分离株被鉴定为伊氏李斯特菌伊氏亚种，其余培养物为伊氏李斯特菌伦敦亚种。分别使用ApaI和SmaI酶切获得了6个和4个PFGE图谱；结合两种酶的结果获得了6个复合图谱。英国伊氏李斯特菌伦敦亚种（从加工虾中分离）的PFGE图谱与该亚种的标准菌株相似，与所有测试的伊氏李斯特菌伊氏亚种不同。大多数分离株（45株中的38株）属于一个图谱，这表明该细菌在英国的群体在遗传上比针对单核细胞增生李斯特菌的类似研究显示的多样性要低得多。
