Côté Daniel, Vitkin I
Opt Express. 2005 Jan 10;13(1):148-63. doi: 10.1364/opex.13.000148.
The concentration determination of optically active species in moderately turbid suspensions is studied both experimentally and with a validated three-dimensional polarization-sensitive Monte Carlo model. It is shown that the orientation of the polarization of the scattered light exhibits a strong dependence on exit position in the side or backscattered directions, but not in the forward direction. In addition, it is shown that the increased path length of photons due to multiple scattering in a 1 cm cuvette filled with forward-peaked scatterers (anisotropy around 0.93) increases the optical rotation by up to 15%, but only for scattering coefficients under 30 cm-1, after which it decreases again. It is concluded that in order to avoid systematic errors in concentration determination of optically-active molecular species in turbid samples, the scattered light in the forward direction should be used.
通过实验和经过验证的三维偏振敏感蒙特卡罗模型，研究了中度浑浊悬浮液中旋光性物质的浓度测定。结果表明，散射光偏振方向在侧向或后向散射方向上对出射位置表现出强烈依赖性，但在正向散射方向上则不然。此外，研究表明，在充满前向散射体（各向异性约为0.93）的1 cm比色皿中，由于多次散射导致光子路径长度增加，使旋光性增加高达15%，但仅适用于散射系数低于30 cm-1的情况，之后旋光性又会下降。得出的结论是，为避免在浑浊样品中旋光性分子物质浓度测定中出现系统误差，应使用正向散射光。
