Witte I, Jacobi H, Juhl-Strauss U
ICBM, Carl von Ossietzky Universität Oldenburg, Germany.
Toxicol Lett. 1996 Sep;87(1):39-45. doi: 10.1016/0378-4274(96)03697-1.
Cytotoxicity screening assays measuring survival, growth, colony forming ability, DNA and protein synthesis in human fibroblasts were tested for their suitability to determine combination effects. Thereby, the dose-response curves of a hydrophilic substance A alone and after pretreatment with a membrane damaging substance B were compared. Substances B were applied at concentrations which did not induce toxic effects in the assays (noec). Synergistic combination effects were demonstrated by reduction of the EC20 value of substances A in the combination in comparison to substances A alone. The following substance pairs (substance B/substance A = membrane damaging/hydrophilic) were tested: n-dodecylbenzenesulfonic acid/2,4-dichlorophenoxyacetic acid, 2,4,6-trichlorophenol/2,4-dichlorophenoxyacetic acid, 1,1,2,2-tetrachloroethane/4-chloroaniline, pentachlorophenol/CrCl3. While survival, growth, and DNA synthesis assays were suitable methods for detecting synergistic combination effects, the growth assay was the most sensitive. Here, all four substance pairs showed synergistic combination effects.
对测量人成纤维细胞存活率、生长、集落形成能力、DNA和蛋白质合成的细胞毒性筛选试验进行了适用性测试，以确定联合效应。由此，比较了亲水性物质A单独作用以及用膜损伤物质B预处理后的剂量反应曲线。物质B的应用浓度在试验中不会诱导毒性效应（无可见效应浓度）。与单独的物质A相比，联合使用时物质A的EC20值降低，证明了协同联合效应。测试了以下物质对（物质B/物质A = 膜损伤/亲水性）：正十二烷基苯磺酸/2,4-二氯苯氧乙酸、2,4,6-三氯苯酚/2,4-二氯苯氧乙酸、1,1,2,2-四氯乙烷/4-氯苯胺、五氯苯酚/CrCl3。虽然存活率、生长和DNA合成试验是检测协同联合效应的合适方法，但生长试验最为敏感。在此，所有四对物质均显示出协同联合效应。
