Valley Justin K, Neale Steven, Hsu Hsan-Yin, Ohta Aaron T, Jamshidi Arash, Wu Ming C
Berkeley Sensor and Actuator Center, Department of Electrical Engineering and Computer Science, University of California Berkeley, Berkeley, CA 94720, USA.
Lab Chip. 2009 Jun 21;9(12):1714-20. doi: 10.1039/b821678a. Epub 2009 Mar 13.
Electroporation is a common technique for the introduction of exogenous molecules across the, otherwise, impermeant cell membrane. Conventional techniques are limited by either low throughput or limited selectivity. Here we present a novel technique whereby we use patterned light to create virtual electrodes which can induce the parallel electroporation of single cells. This technique seamlessly integrates with optoelectronic tweezers to provide a single cell manipulation platform as well. We present evidence of parallel, single cell electroporation using this method through use of fluorescent dyes and dielectrophoretic responses. Additionally, through the use of integrated microfluidic channels, we show that cells remain viable following treatment in the device. Finally, we determine the optimal field dosage to inject propidium iodide into a HeLa cell and maintain cellular viability.
电穿孔是一种将外源分子导入原本不可渗透的细胞膜的常用技术。传统技术受限于低通量或有限的选择性。在此，我们展示了一种新技术，即利用图案化光创建虚拟电极，可诱导单个细胞的平行电穿孔。该技术与光电镊子无缝集成，还提供了一个单细胞操纵平台。我们通过使用荧光染料和介电泳响应，展示了使用此方法进行平行单细胞电穿孔的证据。此外，通过使用集成微流控通道，我们表明细胞在该装置中处理后仍保持活力。最后，我们确定了将碘化丙啶注入HeLa细胞并维持细胞活力的最佳场剂量。
