Peng Hsin-Yin, Yang Chia-Ming, Chen Yu-Ping, Liu Hui-Ling, Chen Tsung-Cheng, Pijanowska Dorota G, Chu Po-Yu, Hsieh Chia-Hsun, Wu Min-Hsien
Institute of Electro-Optical Engineering, Chang Gung University, Taoyuan City 333, Taiwan.
Department of Electronic Engineering, Chang Gung University, Taoyuan City 333, Taiwan.
Biomicrofluidics. 2021 Apr 12;15(2):024109. doi: 10.1063/5.0040910. eCollection 2021 Mar.
To develop a lab on a chip (LOC) integrated with both sensor and actuator functions, a novel two-in-one system based on optical-driven manipulation and sensing in a microfluidics setup based on a hydrogenated amorphous silicon (a-Si:H) layer on an indium tin oxide/glass is first realized. A high-intensity discharge xenon lamp functioned as the light source, a chopper functioned as the modulated illumination for a certain frequency, and a self-designed optical path projected on the digital micromirror device controlled by the digital light processing module was established as the illumination input signal with the ability of dynamic movement of projected patterns. For light-addressable potentiometric sensor (LAPS) operation, alternating current (AC)-modulated illumination with a frequency of 800 Hz can be generated by the rotation speed of the chopper for photocurrent vs bias voltage characterization. The pH sensitivity, drift coefficient, and hysteresis width of the SiN LAPS are 52.8 mV/pH, -3.2 mV/h, and 10.5 mV, respectively, which are comparable to the results from the conventional setup. With an identical two-in-one system, direct current illumination without chopper rotation and an AC bias voltage can be provided to an a-Si:H chip with a manipulation speed of 20 m/s for magnetic beads with a diameter of 1 m. The collection of magnetic beads by this light-actuated AC electroosmosis (LACE) operation at a frequency of 10 kHz can be easily realized. A fully customized design of an illumination path with less decay can be suggested to obtain a high efficiency of manipulation and a high signal-to-noise ratio of sensing. With this proposed setup, a potential LOC system based on LACE and LAPS is verified with the integration of a sensor and an actuator in a microfluidics setup for future point-of-care testing applications.
为了开发一种集成了传感器和执行器功能的芯片实验室（LOC），首先在氧化铟锡/玻璃上基于氢化非晶硅（a-Si:H）层的微流控装置中实现了一种基于光驱动操纵和传感的新型二合一系统。高强度放电氙灯用作光源，斩波器用作特定频率的调制照明，并且在数字光处理模块控制下投射到数字微镜器件上的自行设计的光路被确立为具有投影图案动态移动能力的照明输入信号。对于光寻址电位传感器（LAPS）操作，斩波器的转速可产生频率为800 Hz的交流（AC）调制照明，用于光电流与偏置电压的表征。SiN LAPS的pH灵敏度、漂移系数和滞后宽度分别为52.8 mV/pH、-3.2 mV/h和10.5 mV，与传统装置的结果相当。使用相同的二合一系统，可以在不旋转斩波器且施加交流偏置电压的情况下，以20 m/s的操纵速度向直径为1μm的磁珠的a-Si:H芯片提供直流照明。通过这种频率为10 kHz的光驱动交流电渗（LACE）操作可以轻松实现磁珠的收集。可以提出一种具有较少衰减的照明路径的完全定制设计，以获得高效率的操纵和高信噪比的传感。通过这种提议的装置，基于LACE和LAPS的潜在LOC系统在微流控装置中集成了传感器和执行器，以验证其在未来即时检测应用中的可行性。
