van Oven Carel, Krawczyk Przemek M, Stap Jan, Melo Arline M, Piazzetta Maria H O, Gobbi Angelo L, van Veen Henk A, Verhoeven Jan, Aten Jacob A
Department of Cell Biology and Histology, Center for Microscopical Research, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
Eur Biophys J. 2009 Jul;38(6):721-8. doi: 10.1007/s00249-009-0472-7. Epub 2009 Jun 3.
Localized induction of DNA damage is a valuable tool for studying cellular DNA damage responses. In recent decades, methods have been developed to generate DNA damage using radiation of various types, including photons and charged particles. Here we describe a simple ultrasoft X-ray multi-microbeam system for high dose-rate, localized induction of DNA strand breaks in cells at spatially and geometrically adjustable sites. Our system can be combined with fixed- and live-cell microscopy to study responses of cells to DNA damage.
局部诱导DNA损伤是研究细胞DNA损伤反应的一种有价值的工具。近几十年来，已经开发出了利用包括光子和带电粒子在内的各种类型辐射来产生DNA损伤的方法。在此，我们描述了一种简单的超软X射线多微束系统，用于在空间和几何位置可调节的位点以高剂量率局部诱导细胞中的DNA链断裂。我们的系统可以与固定细胞和活细胞显微镜相结合，以研究细胞对DNA损伤的反应。
