Stirling P, Richmond S
J Gen Microbiol. 1977 May;100(1):31-42. doi: 10.1099/00221287-100-1-31.
The growth of a genital trachoma-inclusion conjunctivitis agent strain of Chlamydia trachomatis in McCoy cells treated with cytochalasin B was studied by quantitative infectivity estimations and by light and electron microscopy. Provided that infection of the monolayer was initiated by centrifuging the infectious particles on to the cells before incubation, this chlamydial strain grew as fast and to as high a titre [approximately 10(7) inclusion-forming units (i.f.u.) per culture] as those chlamydiae which infect cell cultures in vitro without centrifugation. Each i.f.u. inoculated yielded approximately 600 i.f.u., and extracellular infectivity was detected soon after intracellular infectivity appeared. Inclusions were recognized by fluorescent antibody staining techniques early in the developmental cycle when cultures were not infectious and when only reticulate bodies were seen by electron microscopy. Inclusions were recognized in Giemsa-stained preparations examined by dark ground microscopy only when elementary bodies appeared in the inclusions. Iodine staining was not a reliable indicator either of the number of inclusions present or of their infectivity.
通过定量感染性评估以及光学和电子显微镜研究了沙眼衣原体生殖道沙眼-包涵体结膜炎病原体菌株在经细胞松弛素B处理的 McCoy 细胞中的生长情况。前提是在孵育前通过将感染性颗粒离心到细胞上来启动单层细胞的感染,该衣原体菌株的生长速度与那些在体外感染细胞培养物时不进行离心的衣原体一样快,且滴度一样高[每培养物约10(7)个包涵体形成单位(i.f.u.)]。接种的每个i.f.u.产生约600个i.f.u.,并且在细胞内感染性出现后不久就检测到细胞外感染性。当培养物无感染性且通过电子显微镜仅能看到网状体时,在发育周期早期通过荧光抗体染色技术可识别包涵体。仅当包涵体中出现原体时,在暗视野显微镜检查的吉姆萨染色制剂中才能识别包涵体。碘染色无论是对于存在的包涵体数量还是其感染性都不是一个可靠的指标。