Knight S T, Lee S H, Davis C H, Moorman D R, Hodinka R L, Wyrick P B
Department of Microbiology and Immunology, UNC School of Medicine, Chapel Hill 27514.
Sex Transm Dis. 1987 Jul-Sep;14(3):165-73. doi: 10.1097/00007435-198707000-00009.
Nonoxynol-9, a nonionic detergent and active ingredient in spermicidal contraceptives, has been reported to have anti-chlamydial properties. However, in this study exposure of elementary bodies of Chlamydia trachomatis serovar E to nonoxynol-9 (12.5-10,000 micrograms/ml) had no effect on chlamydial infectivity. In contrast, uninfected McCoy cells incubated with increasing concentrations of nonoxynol-9 over 72 h displayed dose-related cytotoxicity. When infected McCoy cells were exposed to nonoxynol-9, the developing chlamydial inclusions did not stain with iodine even though they were similar in number and appearance to the inclusions in unexposed, infected monolayers. Transmission electron microscopy of nonoxynol-treated, infected cells revealed apparent damage to the inclusion membrane and reticulate bodies within. The infectivity of the chlamydiae in the iodine-negative inclusions on subpassage was only 0.3%. We conclude that the primary action of nonoxynol-9 is on the McCoy cell and that there may be secondary effects on the intracellular parasite.
壬苯醇醚 - 9是一种非离子型去污剂，也是杀精避孕药中的活性成分，据报道具有抗衣原体特性。然而，在本研究中，沙眼衣原体血清型E的原体暴露于壬苯醇醚 - 9（12.5 - 10000微克/毫升）对衣原体感染性没有影响。相比之下，未感染的 McCoy 细胞在72小时内与浓度不断增加的壬苯醇醚 - 9孵育，显示出剂量相关的细胞毒性。当感染的 McCoy 细胞暴露于壬苯醇醚 - 9时，正在发育的衣原体包涵体即使在数量和外观上与未暴露的感染单层中的包涵体相似，也不会被碘染色。对经壬苯醇醚处理的感染细胞进行透射电子显微镜检查发现，包涵体膜和内部的网状体有明显损伤。传代时碘阴性包涵体中的衣原体感染性仅为0.3%。我们得出结论，壬苯醇醚 - 9的主要作用是对 McCoy 细胞，并且可能对细胞内寄生虫有次要影响。
