Department of Family and Child Nursing, University of Washington, Seattle, WA 98195, USA.
Biol Res Nurs. 2010 Jan;11(3):288-92. doi: 10.1177/1099800409337330. Epub 2009 Jun 4.
The purpose of this study was to assess feasibility and acceptability of using a diaper pad for collection of in-home infant urinary samples and to test the accuracy of diaper pad extraction for 6-sulfatoxymelatonin and creatinine, which was used to correct assay results for urinary volume. To assess feasibility and acceptability, urine samples from 20 infants were collected over a 24-hr day using a cotton pad inserted in the diaper. The accuracy of diaper pad extraction was evaluated in the laboratory using urine samples collected from 11 adult volunteers and assayed using enzyme immunosorbent assay (EIA). Urine samples were divided, one aliquot was assayed without extraction, and one aliquot was instilled into a diaper pad, extracted, and assayed. Mothers found diaper pad collection acceptable and easy to perform. Of 144 infant urinary samples obtained in the home environment, 59% were usable for assay purposes, and the remaining either were contaminated with stool or were of insufficient volume. While creatinine values from diaper pad extracted and nonextracted samples were highly correlated (r(2) = .947), those of creatinine-corrected 6-sulfatoxymelatonin were not (r(2) = .216). Diaper pad collection procedures altered 6-sulfatoxymelatonin values. Implications for measurement of urinary biochemical substances and statistical analysis are discussed.
本研究旨在评估使用尿布垫收集家庭中婴儿尿液样本的可行性和可接受性，并测试尿布垫提取 6-硫酸褪黑素和肌酐的准确性，以校正尿液量的分析结果。为了评估可行性和可接受性，使用插入尿布中的棉垫收集了 20 名婴儿的 24 小时尿液样本。使用从 11 名成人志愿者收集的尿液样本在实验室中评估了尿布垫提取的准确性，并使用酶联免疫吸附测定（EIA）进行了测定。将尿液样本分成两部分，一部分未经提取进行测定，另一部分注入尿布垫中提取并进行测定。母亲们发现尿布垫收集是可接受且易于操作的。在家环境中获得的 144 份婴儿尿液样本中，59%可用于测定目的，其余的要么被粪便污染，要么体积不足。虽然尿布垫提取和未提取样本的肌酐值高度相关（r² =.947），但肌酐校正的 6-硫酸褪黑素的相关系数却不是（r² =.216）。尿布垫收集程序改变了 6-硫酸褪黑素的值。讨论了对尿液生化物质的测量和统计分析的影响。
