在乳清中生长时诱导产生的来自牛乳腺炎的金黄色葡萄球菌的类体内抗原表面特性。

In vivo-like antigenic surface properties of Staphylococcus aureus from bovine mastitis induced upon growth in milk whey.

作者信息

Mamo W, Fröman G

机构信息

Department of Veterinary Microbiology, Swedish University of Agricultural Sciences.

出版信息

Microbiol Immunol. 1994;38(10):801-4. doi: 10.1111/j.1348-0421.1994.tb01860.x.

DOI:10.1111/j.1348-0421.1994.tb01860.x

PMID:7869958

Abstract

Antigenic surface properties of Staphylococcus aureus strains grown in milk whey were compared with TSB-grown bacteria using immuno-gold electron microscopy. It is shown that colloidal gold (CG) particles coated with polyclonal antibody raised against Staphylococcus aureus surface antigen expressed in vivo bound to the surface of S. aureus strain F1440 grown in milk whey, but not to homologous bacteria grown in TSB. S. aureus strains grown in milk whey agglutinated in the presence of the polyclonal antibody, whereas the corresponding bacteria grown in TSB did not agglutinate. Immuno-gold particles did not bind to milk whey-grown bacteria treated with periodate. Periodate-treated milk whey-grown bacteria did not agglutinate in the presence of the polyclonal antibody, whereas periodate treatment had no effect on TSB-grown bacteria.

摘要

使用免疫金电子显微镜，将在乳清中生长的金黄色葡萄球菌菌株的抗原表面特性与在胰酪大豆肉汤（TSB）中生长的细菌进行了比较。结果表明，包被有针对体内表达的金黄色葡萄球菌表面抗原产生的多克隆抗体的胶体金（CG）颗粒，能与在乳清中生长的金黄色葡萄球菌F1440菌株的表面结合，但不与在TSB中生长的同源细菌结合。在乳清中生长的金黄色葡萄球菌菌株在多克隆抗体存在下发生凝集，而在TSB中生长的相应细菌则不凝集。免疫金颗粒不与经高碘酸盐处理的在乳清中生长的细菌结合。经高碘酸盐处理的在乳清中生长的细菌在多克隆抗体存在下不凝集，而高碘酸盐处理对在TSB中生长的细菌没有影响。