Phillips M D, Flynn M G, McFarlin B K, Stewart L K, Timmerman K L, Ji H
Department of Kinesiology, Texas Christian University, Fort Worth, TX 76123, USA.
Int J Sports Med. 2008 Feb;29(2):102-9. doi: 10.1055/s-2007-965115. Epub 2007 Sep 13.
To examine the influence of acute resistive exercise and "hormone status" on cytokine profile, 35 postmenopausal women (72 +/- 6.2 yr) underwent a moderate-high-intensity resistive exercise bout or rested. There were 4 groups: no hormone replacement (NHR, n = 9), hormone replacement (HRT, n = 12), selective estrogen receptor modulator (SER, n = 7), or resting control (no hormone replacement, CON, n = 7). NHR, HRT, and SER exercised (3 sets, 10 exercises @ 80 % 1RM). Blood was collected pre-exercise (PR), postexercise (PO), and two hours (2H) postexercise (same times for CON). Blood was diluted 1 : 10 in culture medium and incubated (37 degrees C, 5 % CO2, 24 h) with lipopolysaccharide (LPS, 25 microg . ml (-1)). Serum and supernatant from LPS-stimulated blood were analyzed for IL-6, IL-1 beta, and TNF-alpha using ELISA. Resistive exercise increased PO serum IL-6, and PO LPS-stimulated IL-6 and IL-1 beta in the exercise groups (HRT, NHR, SER collapsed; EX, n = 28). LPS-stimulated IL-1 beta remained elevated at 2H in EX and was significantly higher than PR in CON at 2H. Expressed per monocyte, EX had significantly lower IL-1 beta and TNF-alpha LPS-stimulated production at PO and 2H compared to CON, indicating an exercise-induced blunting of an apparent diurnal response on cytokine production. In postmenopausal women, acute resistive exercise increased circulating IL-6, but reversed an apparent diurnal increase in LPS-stimulated IL-1 beta and TNF-alpha production with no influence of hormone replacement or raloxifene.
为研究急性抗阻运动和“激素状态”对细胞因子谱的影响，35名绝经后女性（72±6.2岁）进行了一次中高强度抗阻运动或休息。分为4组：未进行激素替代（NHR，n = 9）、激素替代（HRT，n = 12）、选择性雌激素受体调节剂（SER，n = 7）或休息对照（未进行激素替代，CON，n = 7）。NHR、HRT和SER组进行运动（3组，10项练习，强度为80% 1RM）。在运动前（PR）、运动后（PO）和运动后两小时（2H）采集血液（CON组在相同时间采集）。血液在培养基中按1:10稀释，并与脂多糖（LPS，25μg·ml⁻¹）一起孵育（37℃，5% CO₂，24小时）。使用ELISA分析LPS刺激血液后的血清和上清液中的IL-6、IL-1β和TNF-α。抗阻运动使运动组的PO血清IL-6以及PO LPS刺激后的IL-6和IL-1β升高（HRT、NHR、SER合并；EX，n = 28）。LPS刺激后的IL-1β在EX组的2H时仍保持升高，且在2H时显著高于CON组的PR水平。与CON组相比，EX组在PO和2H时每单核细胞的LPS刺激后IL-1β和TNF-α产生显著降低，表明运动诱导了细胞因子产生的明显昼夜反应减弱。在绝经后女性中，急性抗阻运动增加了循环IL-6，但逆转了LPS刺激后IL-1β和TNF-α产生的明显昼夜增加，且不受激素替代或雷洛昔芬的影响。
