Piekarski Nadine, Hobbs Theodore R, Jacob Darla, Schwartz Tiah, Burch Fernanda C, Mishler Emily C, Jensen Jared V, Krieg Sacha A, Hanna Carol B
Division of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Oregon Health & Science University, Beaverton, OR 97006, USA.
Animal Resources & Research Support, Oregon National Primate Research Center, Oregon Health & Science University, Beaverton, OR 97006, USA.
Animals (Basel). 2023 Sep 26;13(19):3017. doi: 10.3390/ani13193017.
Obtaining quality oocytes is a prerequisite for ART-based studies. Here we describe a method for transabdominal ultrasound-guided (US) oocyte retrieval in rhesus macaques () and compare it to the standard surgical approach using laparoscopy (LAP). We analyzed oocyte yield from six continuous reproductive seasons (2017-2023) that included = 177 US-guided and = 136 laparoscopic oocyte retrievals. While the ultrasound-guided technique retrieved significantly fewer oocytes on average (LAP: 40 ± 2 vs. US: 27 ± 1), there was no difference in the number of mature metaphase II oocytes (MII) between the two techniques (LAP: 17 ± 1 vs. US: 15 ± 1). We show that oocytes retrieved by the ultrasound-guided approach fertilize at the same rates as those obtained via the laparoscopic procedure (LAP Fert Rate: 84% ± 2% vs. US Fert Rate: 83% ± 2%). In conclusion, minimally invasive ultrasound-guided oocyte retrieval improves animal welfare while delivering equivalent numbers of mature oocytes, which are ideal for ART. Furthermore, we show that oocyte competency, as represented by fertilization rate, is not affected by retrieval technique. Therefore, the Oregon National Primate Research Center (ONPRC) has adopted the ultrasound-guided approach as the standard technique for oocyte retrieval.
获取高质量的卵母细胞是基于辅助生殖技术（ART）研究的前提条件。在此，我们描述了一种在恒河猴中经腹超声引导下取卵的方法，并将其与使用腹腔镜检查（LAP）的标准手术方法进行比较。我们分析了六个连续生殖季节（2017 - 2023年）的卵母细胞产量，其中包括177次超声引导下取卵和136次腹腔镜取卵。虽然超声引导技术平均获取的卵母细胞数量明显较少（LAP：40 ± 2个 vs. US：27 ± 1个），但两种技术获得的成熟中期II期卵母细胞（MII）数量没有差异（LAP：17 ± 1个 vs. US：15 ± 1个）。我们发现，超声引导方法获取的卵母细胞与通过腹腔镜手术获取的卵母细胞受精率相同（LAP受精率：84% ± 2% vs. US受精率：83% ± 2%）。总之，微创超声引导下取卵在提供等量成熟卵母细胞的同时提高了动物福利，这些卵母细胞是ART的理想选择。此外，我们表明，以受精率表示的卵母细胞能力不受取卵技术的影响。因此，俄勒冈国家灵长类动物研究中心（ONPRC）已采用超声引导方法作为取卵的标准技术。
