Ndhlala A R, Amoo S O, Stafford G I, Finnie J F, Van Staden J
Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, Scottsville 3209, South Africa.
J Ethnopharmacol. 2009 Jul 30;124(3):404-8. doi: 10.1016/j.jep.2009.05.037. Epub 2009 Jun 6.
In recent times, many products ranging from aloe drinks to aloe gels, powders, capsules, and creams have appeared on the commercial market prepared from different aloe species including Aloe barberae. These products are used in ethnomedicine to treat various conditions including gastrointestinal disorders, insect bites, skin burns and other skin injuries by traditional communities.
This study was aimed at evaluating the antibacterial, antifungal and anti-inflammatory activities as well as genotoxic effects of different extracts of Aloe barberae.
Organic and water extracts of the upper stem, young bark, mature bark, leaves and roots of the South African tree aloe (Aloe barberae) were evaluated for their antimicrobial [gram-positive (Bacillus subtilis and Staphylococcus aureus), gram-negative (Escherichia coli and Klebsiella pneumoniae) bacteria as well as the fungus Candida albicans], anti-inflammatory (COX-1 and COX-2) and mutagenic properties (Ames test). Thin layer chromatography (TLC) was used to compare the phytochemical profiles of different extracts of Aloe barberae.
The petroleum ether (PE) and dichloromethane (DCM) extracts of the mature bark, leaves and roots exhibited good activity against all the bacteria and fungus Candida albicans with minimum inhibitory concentrations (MIC) ranging from 0.195 to 1.56 mg/ml. All the PE extracts evaluated showed a high activity (>70%) in both COX-1 and COX-2 assays. Apart from the organic extracts of the root with consistently good activity (>70%), all the remaining extracts showed moderate activity (40-69%) in COX-1 assay. The PE extracts also showed a dose dependent increase in activity. Ultraviolet (UV) spectrum of the leaves and root EtOH extracts indicated the presence of compounds that could absorb UV light (wavelength: 190-820 nm). None of the extracts had a mutagenic effect in the Salmonella/microsome assay against a tester strain, TA98.
Activity observed in the bark, leaves and roots of Aloe barberae validates its use in commercial herbal products, ethnobotany and ethnoveterinary medicine by South African communities and small scale farmers to treat various conditions.
近年来，许多产品，从芦荟饮料到芦荟凝胶、粉末、胶囊和乳膏，已出现在商业市场上，这些产品由包括好望角芦荟在内的不同芦荟品种制成。传统社区在民族医学中使用这些产品来治疗各种病症，包括胃肠道疾病、昆虫叮咬、皮肤烧伤和其他皮肤损伤。
本研究旨在评估好望角芦荟不同提取物的抗菌、抗真菌和抗炎活性以及遗传毒性作用。
对南非树芦荟（好望角芦荟）的上部茎、幼树皮、成熟树皮、叶和根的有机提取物和水提取物进行抗菌活性评估[革兰氏阳性菌（枯草芽孢杆菌和金黄色葡萄球菌）、革兰氏阴性菌（大肠杆菌和肺炎克雷伯菌）以及白色念珠菌]、抗炎活性（COX - 1和COX - 2）和致突变特性（艾姆斯试验）。采用薄层色谱法（TLC）比较好望角芦荟不同提取物的植物化学图谱。
成熟树皮、叶和根的石油醚（PE）提取物和二氯甲烷（DCM）提取物对所有细菌和白色念珠菌均表现出良好活性，最低抑菌浓度（MIC）范围为0.195至1.56 mg/ml。所有评估的PE提取物在COX - 1和COX - 2试验中均表现出高活性（>70%）。除根部有机提取物始终具有良好活性（>70%）外，其余所有提取物在COX - 1试验中均表现出中等活性（40 - 69%）。PE提取物的活性也呈现剂量依赖性增加。叶和根乙醇提取物的紫外（UV）光谱表明存在可吸收紫外光的化合物（波长：190 - 820 nm）。在针对测试菌株TA98的沙门氏菌/微粒体试验中，没有一种提取物具有致突变作用。
在好望角芦荟的树皮、叶和根中观察到的活性证实了南非社区和小规模农民在商业草药产品、民族植物学和民族兽医学中使用它来治疗各种病症的合理性。
