Research Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, Scottsville 3209, South Africa.
J Ethnopharmacol. 2013 Mar 7;146(1):173-9. doi: 10.1016/j.jep.2012.12.026. Epub 2012 Dec 28.
Inflammation is a major risk factor for various human diseases including venereal diseases, often resulting in treatment complications. Plants have been traditionally used for treatment of many different diseases and have been successfully proven to be an alternative source in treatment of infectious diseases.
This study was aimed at evaluating the anti-inflammatory activities and the mutagenic properties of 12 medicinal plants used by the Venda people against venereal and related diseases.
The plants were evaluated for their anti-inflammatory activity against the cyclooxygenase (COX-1 and -2) enzymes and genotoxicity using the Ames test, with and without S9 (metabolic activation) against Salmonella typhimurium tester strain TA98.
DCM and PE extracts of Adansonia digitata bark, Bolusanthus speciosus bark, Pterocarpus angolensis bark and Pappea capensis leaves and EtOH and water extracts of Bolusanthus speciosus stem and Ekebergia capensis bark showed the best anti-inflammatory activity in both COX-1 and -2 assays at 250 μg/ml. These were further evaluated at three other concentrations (31.25, 62.5, and 125 μg/ml) to determine IC50 values. Water extracts of Ekebergia capensis bark showed the best IC50 value towards COX-1. The Ames test revealed that all plant extracts were non-mutagenic towards Salmonella typhimurium strain TA98 except for Elephantorrhiza burkei and Ekebergia capensis that showed weak mutagenicity.
The active plants may offer a new source of chemicals for the effective treatment of anti-inflammatory conditions related to venereal diseases.
炎症是包括性病在内的多种人类疾病的主要风险因素，常导致治疗并发症。植物在传统上被用于治疗许多不同的疾病，并已成功证明是治疗传染病的替代来源。
本研究旨在评估文达人用于治疗性病和相关疾病的 12 种药用植物的抗炎活性和致突变特性。
使用 Ames 试验，分别在有和没有 S9（代谢激活）的情况下，评估植物对环氧化酶（COX-1 和 COX-2）的抗炎活性和致突变性，采用鼠伤寒沙门氏菌测试菌株 TA98。
DCM 和 PE 提取物的猴面包树树皮、博鲁斯安特斯斯比奥修斯树皮、紫檀树皮和卡彭西亚叶，以及博鲁斯安特斯斯比奥修斯茎和埃克贝加西亚树皮的 EtOH 和水提取物，在 250μg/ml 时在 COX-1 和 COX-2 测定中显示出最佳的抗炎活性。进一步在另外三个浓度（31.25、62.5 和 125μg/ml）下进行评估，以确定 IC50 值。埃克贝加西亚树皮的水提取物对 COX-1 显示出最佳的 IC50 值。Ames 试验表明，除了大象根和埃克贝加西亚外，所有植物提取物对鼠伤寒沙门氏菌 TA98 均无致突变性，而大象根和埃克贝加西亚显示出弱致突变性。
活性植物可能为治疗与性病相关的抗炎病症提供新的化学物质来源。
