Pomeroy K A, Paul P S, Weber A F, Sorensen D K, Johnson D W
J Natl Cancer Inst. 1977 Jul;59(1):281-3. doi: 10.1093/jnci/59.1.281.
Peripheral blood lymphocytes from 3 cows with persistent lymphocytosis were separated on nylon wool columns into nylon wool-adherent and nonadherent populations. The percentage of cells coated with surface immunoglobulin (B-cells) and the frequency of lymphocytic nuclear pockets in each sub-population were then determined. In each case the adherent population consisted predominantly of B-cells with an increased nuclear pocket frequency, whereas the nonadherent cells were 98.99% negative for surface immunoglobulin (non-B-cells) and contained essentially no nuclear pockets. These findings provided additional evidence that the B-subpopulation of cells was highly involved in bovine leukemia oncogenesis.
从3头患有持续性淋巴细胞增多症的奶牛采集外周血淋巴细胞，通过尼龙毛柱将其分离为尼龙毛黏附细胞群和非黏附细胞群。然后测定每个亚群中表面免疫球蛋白包被细胞（B细胞）的百分比以及淋巴细胞核袋的频率。在每种情况下，黏附细胞群主要由核袋频率增加的B细胞组成，而非黏附细胞表面免疫球蛋白呈98.99%阴性（非B细胞），且基本不含核袋。这些发现进一步证明细胞的B亚群高度参与牛白血病的肿瘤发生过程。
