Paul P S, Pomeroy K A, Johnson D W, Muscoplat C C, Handwerger B S, Soper F F, Sorensen D K
Am J Vet Res. 1977 Jun;38(6):873-6.
Bovine peripheral blood lymphocytes from a cow with persistent lymphocytosis were separated on nylon wool columns into nylon-adherent and nonadherent populations. Nylon-adherent cells were highly enriched for surface immunoglobulin (SIg) bearing B lymphocytes (95.5%) and nonadherent cells for SIg negative non-B cells, presumably T lymphocytes (96.3%). The B lymphocytes were found to be the major producers for bovine leukemia virus. A total of 39% of the B-enriched cells, surviving after 72 hours in culture, produced bovine leukemia virus as compared with 0.5% of the non-B cells.
从患有持续性淋巴细胞增多症的奶牛中获取牛外周血淋巴细胞，通过尼龙毛柱将其分离为尼龙黏附细胞群和非黏附细胞群。尼龙黏附细胞高度富集了带有表面免疫球蛋白（SIg）的B淋巴细胞（95.5%），而非黏附细胞则富含SIg阴性的非B细胞，推测为T淋巴细胞（96.3%）。研究发现B淋巴细胞是牛白血病病毒的主要产生者。在培养72小时后存活的富含B细胞的细胞群中，共有39%产生了牛白血病病毒，相比之下，非B细胞中这一比例为0.5%。
