Paul P S, Pomeroy K A, Castro A E, Johnson D W, Muscoplat C C, Sorensen D K
J Natl Cancer Inst. 1977 Oct;59(4):1269-72. doi: 10.1093/jnci/59.4.1269.
Bovine peripheral blood lymphocytes (PBL's) from 3 cows and 1 steer infected with bovine leukemia virus (BLV) were separated by fractionation through nylon wool columns into nylon-adherent and nonadherent cell populations. Nylon-adherent cells were enriched in B-lymphocytes, as determined by the presence of surface membrane immunoglobulins (slg), whereas nylon-nonadherent cells or "non-B-lymphocytes" contained few slg-bearing cells. PBL's and separated B- and non-B-lymphocyte populations were assayed for the presence of BLV by the induction of syncytia in bovine embryonic spleen cells. PBL's and B-lymphocyte populations both produced many syncytia, whereas non-B-lymphocytes yielded few or no syncytia. The specificity of syncytia formation by anti-BLV serum. PBL's from 2 control animals were negative for syncytia induction. This study presents further evidence that B-lymphocytes are the target cells for BLV infection.
从3头奶牛和1头公牛身上采集感染牛白血病病毒（BLV）的牛外周血淋巴细胞（PBL），通过尼龙棉柱分级分离，分为尼龙黏附细胞群和非黏附细胞群。通过表面膜免疫球蛋白（slg）的存在确定，尼龙黏附细胞富含B淋巴细胞，而尼龙非黏附细胞或“非B淋巴细胞”含有的携带slg的细胞很少。通过在牛胚胎脾细胞中诱导形成多核巨细胞，检测PBL以及分离出的B淋巴细胞群和非B淋巴细胞群中BLV的存在情况。PBL和B淋巴细胞群都产生了许多多核巨细胞，而非B淋巴细胞产生的多核巨细胞很少或没有。抗BLV血清诱导多核巨细胞形成的特异性。来自2只对照动物的PBL诱导多核巨细胞形成呈阴性。这项研究进一步证明B淋巴细胞是BLV感染的靶细胞。
