胃癌中HACE1的甲基化

Methylation of HACE1 in gastric carcinoma.

作者信息

Sakata Makiko, Kitamura Yo-Hei, Sakuraba Kazuma, Goto Tetsuhiro, Mizukami Hiroki, Saito Mitsuo, Ishibashi Kazuyoshi, Kigawa Gaku, Nemoto Hiroshi, Sanada Yutaka, Hibi Kenji

机构信息

Department of Surgery, Showa University Fujigaoka Hospital, Aoba-ku, Yokohama 227-8501, Japan.

出版信息

Anticancer Res. 2009 Jun;29(6):2231-3.

PMID:19528486

Abstract

BACKGROUND

We recently examined the methylation status of the HACE1 gene in primary carcinomas derived from 32 patients with colorectal cancer. A significant increase was observed in the maximal tumor size of the tumors with methylated HACE1 (p=0.0304). Moreover, a trend was shown toward preferentially developing lymph node metastasis in the carcinomas with methylated HACE1 (p=0.0612), suggesting that HACE1 might present a malignant potential in colorectal cancer. These results prompted us to examine the methylation status of the HACE1 gene in gastric carcinomas.

MATERIALS AND METHODS

The methylation status of the HACE1 gene was examined in primary carcinomas and the corresponding normal tissues derived from 34 patients with gastric carcinoma using quantitative methylation-specific PCR (qMSP) and the correlation between the methylation status and the clinicopathological findings was evaluated.

RESULTS

An aberrant methylation of the HACE1 gene was detected in 9 out of 34 (26%) primary gastric carcinomas. Subsequently, clinicopathological data were tested for correlation with the methylation score. A significant difference was observed in patient gender (p=0.0429).

CONCLUSION

HACE1 was frequently methylated in gastric carcinoma derived from male patients.

摘要

背景

我们最近检测了32例结直肠癌患者原发癌中HACE1基因的甲基化状态。观察到HACE1甲基化的肿瘤最大肿瘤大小显著增加（p = 0.0304）。此外，HACE1甲基化的癌显示出优先发生淋巴结转移的趋势（p = 0.0612），这表明HACE1可能在结直肠癌中呈现恶性潜能。这些结果促使我们检测胃癌中HACE1基因的甲基化状态。