Department of Physiology, Medicine and Dentistry School, University of the Basque Country, Leioa, Spain.
Department of Obstetrics and Gynecology, Cruces Hospital, University of the Basque Country, Leioa, Spain.
Fertil Steril. 2010 Sep;94(4):1279-1286. doi: 10.1016/j.fertnstert.2009.05.028. Epub 2009 Jun 21.
To evaluate the serum oxidizability and antioxidant status in women undergoing an in vitro fertilization (IVF) cycle and to assess the possible relationship of the oxidizability indexes with the pregnancy rate.
Prospective, longitudinal study.
Public university and public university hospital.
PATIENT(S): Systematically recruited cohort of 125 women undergoing either IVF or intracytoplasmic sperm injection (ICSI).
INTERVENTION(S): Serum samples were collected before the beginning of the use of gonadotropins (basal) and the day of human chorionic gonadotropin (hCG) administration (final) during an IVF cycle.
MAIN OUTCOME MEASURE(S): The Cu2+-induced serum oxidation in terms of the oxidation rate in the lag (Vlag) and propagation (Vmax) phases and the time at which the oxidation rate is maximal (tmax), and measurements of serum total antioxidant activity (TAA), tocopherol, hydrophilic antioxidants, malondialdehyde, and nitric oxide.
RESULT(S): Albumin, urate, bilirubin, alpha-tocopherol and gamma-tocopherol, TAA, and tmax statistically significantly decreased after the IVF cycle. Conception cycles were associated with a serum more prone to oxidation compared with nonconception cycles. In multivariate logistic regression analysis, the difference (final-basal) of the oxidation index Vlag (OR 1.394) and the body mass index (OR 0.785) were independent predictors of pregnancy.
CONCLUSION(S): Treatment with IVF induces the production of reactive oxygen species (ROS), which is reflected in a serum less protected against oxidation. The results also suggest a role for ROS in the occurrence of conception in IVF.
评估体外受精（IVF）周期中女性的血清氧化能力和抗氧化状态，并评估氧化指数与妊娠率的可能关系。
前瞻性、纵向研究。
公立大学和公立医院。
接受 IVF 或卵胞浆内单精子注射（ICSI）的 125 名系统性招募的女性队列。
在 IVF 周期中，在开始使用促性腺激素之前（基础）和人绒毛膜促性腺激素（hCG）给药当天（最终）采集血清样本。
Cu2+诱导的血清氧化速率，包括滞后（Vlag）和传播（Vmax）阶段的氧化速率以及达到最大氧化速率的时间（tmax），以及血清总抗氧化活性（TAA）、生育酚、亲水性抗氧化剂、丙二醛和一氧化氮的测量。
白蛋白、尿酸、胆红素、α-生育酚和γ-生育酚、TAA 和 tmax 在 IVF 周期后统计学显著降低。与非妊娠周期相比，妊娠周期的血清更容易氧化。在多变量逻辑回归分析中，氧化指数 Vlag 的差异（最终-基础）（OR 1.394）和体重指数（OR 0.785）是妊娠的独立预测因子。
IVF 治疗会产生活性氧（ROS），这反映在血清中抗氧化能力降低。结果还表明，ROS 在 IVF 中妊娠的发生中起作用。
