Sunar Ulas, Makonnen Sosina, Zhou Chao, Durduran Turgut, Yu Guoqiang, Wang Hsing-Wen, Lee William M, Yodh Arjun G
Opt Express. 2007 Nov 12;15(23):15507-16. doi: 10.1364/oe.15.015507.
Diffuse optical methods were used to monitor two different therapies in K1735 malignant mouse melanoma tumor models: anti-vascular therapy and radiation therapy. Anti-vascular therapy induced acute variation in hemodynamic parameters within an hour, and radiation therapy induced longitudinal changes within 2 weeks. During anti-vascular therapy, the drug Combretastatin A-4 3-O-Phosphate (CA4P, 2.5 mg/200 mul PBS/mouse) significantly decreased tissue blood flow (65%) and blood oxygenation (38%) one hour after injection. In the longitudinal study, single-fraction ionizing radiation (12 Gy x 1) induced significant reduction of tissue blood flow (36%) and blood oxygenation (24%) 14 days after radiation. The results correlated well with contrast enhanced ultrasound, tumor histology, and a nitroimidazole hypoxia marker (EF5). The research provides further evidence that noninvasive diffuse optical spectroscopies can be useful tools for monitoring cancer therapy in vivo.
采用漫射光学方法在K1735恶性小鼠黑色素瘤肿瘤模型中监测两种不同的治疗方法：抗血管治疗和放射治疗。抗血管治疗在一小时内引起血流动力学参数的急性变化，而放射治疗在两周内引起纵向变化。在抗血管治疗期间，注射药物磷酸康普瑞汀A - 4（CA4P，2.5 mg/200 μl PBS/小鼠）一小时后，组织血流量（65%）和血液氧合（38%）显著降低。在纵向研究中，单次分割电离辐射（12 Gy×1）在辐射后14天引起组织血流量（36%）和血液氧合（24%）显著降低。结果与超声造影、肿瘤组织学以及硝基咪唑类缺氧标志物（EF5）相关性良好。该研究进一步证明，无创漫射光学光谱学可作为体内监测癌症治疗的有用工具。
