Alandejani Talal, Marsan Joseph, Ferris Wendy, Slinger Robert, Chan Frank
Department of Otolaryngology, University of Ottawa, and Childrens Hospital of Eastern Ontario, Ottawa, Ontario, Canada.
Otolaryngol Head Neck Surg. 2009 Jul;141(1):114-8. doi: 10.1016/j.otohns.2009.01.005. Epub 2009 Mar 9.
Biofilms formed by Pseudomonas aeruginosa (PA) and Staphylococcus aureus (SA) have been shown to be an important factor in the pathophysiology of chronic rhinosinusitis (CRS). As well, honey has been used as an effective topical antimicrobial agent for years. Our objective is to determine the in vitro effect of honey against biofilms produced by PA and SA.
In vitro testing of honey against bacterial biofilms.
We used a previously established biofilm model to assess antibacterial activity of honey against 11 methicillin-susceptible SA (MSSA), 11 methicillin-resistant SA (MRSA), and 11 PA isolates. Honeys were tested against both planktonic and biofilm-grown bacteria.
Honey was effective in killing 100 percent of the isolates in the planktonic form. The bactericidal rates for the Sidr and Manuka honeys against MSSA, MRSA, and PA biofilms were 63-82 percent, 73-63 percent, and 91-91 percent, respectively. These rates were significantly higher (P<0.001) than those seen with single antibiotics commonly used against SA.
Honey, which is a natural, nontoxic, and inexpensive product, is effective in killing SA and PA bacterial biofilms. This intriguing observation may have important clinical implications and could lead to a new approach for treating refractory CRS.
铜绿假单胞菌（PA）和金黄色葡萄球菌（SA）形成的生物膜已被证明是慢性鼻-鼻窦炎（CRS）病理生理学中的一个重要因素。此外，蜂蜜多年来一直被用作一种有效的局部抗菌剂。我们的目的是确定蜂蜜对PA和SA产生的生物膜的体外作用。
蜂蜜对细菌生物膜的体外测试。
我们使用先前建立的生物膜模型来评估蜂蜜对11株甲氧西林敏感SA（MSSA）、11株甲氧西林耐药SA（MRSA）和11株PA分离株的抗菌活性。对浮游菌和生物膜生长的细菌都进行了蜂蜜测试。
蜂蜜能有效杀死100%的浮游形式的分离株。枣椰蜂蜜和麦卢卡蜂蜜对MSSA、MRSA和PA生物膜的杀菌率分别为63%-82%、73%-63%和91%-91%。这些杀菌率显著高于（P<0.001）常用的针对SA的单一抗生素的杀菌率。
蜂蜜是一种天然、无毒且廉价的产品，能有效杀死SA和PA细菌生物膜。这一有趣的观察结果可能具有重要的临床意义，并可能导致一种治疗难治性CRS的新方法。
