Van R L, Roncari D A
Cell Tissue Res. 1977 Jul 11;181(2):197-203. doi: 10.1007/BF00219980.
The possible existence of adipocyte precursors in adult rat adipose tissue was investigated. Cells were isolated from the stromal fraction of adipose tissue and were grown in culture. Skin fibroblasts were used as controls. The stromal fraction cells were initially fusiform and proliferated; in culture, they accumulated lipid inclusions, became rounder and acquired an eccentric nucleus. In contrast, the skin fibroblasts from the same rat grown under identical culture conditions, did not exhibit any appreciable lipid accumulation. The doubling time for both the stromal fraction cells and skin fibroblasts was 40-60 h. At confluency, the stromal fraction cells contained 5-7 times more glyceride-glycerol than skin fibroblasts. Thus, adipose tissue of adult rats contains cells with the potential to proliferate and acquire morphological characteristics similar to those of adipocytes.
对成年大鼠脂肪组织中脂肪细胞前体的可能存在情况进行了研究。从脂肪组织的基质部分分离细胞并进行培养。使用皮肤成纤维细胞作为对照。基质部分的细胞最初呈梭形并增殖；在培养过程中，它们积累脂质包涵体，变得更圆并获得一个偏心核。相比之下，在相同培养条件下生长的同一只大鼠的皮肤成纤维细胞没有表现出任何明显的脂质积累。基质部分细胞和皮肤成纤维细胞的倍增时间均为40 - 60小时。汇合时，基质部分细胞所含的甘油三酯甘油比皮肤成纤维细胞多5 - 7倍。因此，成年大鼠的脂肪组织含有具有增殖潜力并获得与脂肪细胞相似形态特征的细胞。
