Yang Wei, Xue Hong, Li Wei, Zhang Jinli, Jiang Shaoyi
Department of Chemical Engineering, University of Washington, Box 351750, Seattle, Washington 98195, USA.
Langmuir. 2009 Oct 6;25(19):11911-6. doi: 10.1021/la9015788.
Human blood serum and plasma pose significant challenges to blood-contacting devices and implanted materials because of their high nonspecific adsorption onto surfaces. In this work, we investigated nonspecific protein adsorption from single protein solutions and complex media such as undiluted human blood serum and plasma onto poly(carboxybetaine acrylamide) (polyCBAA)-grafted surfaces at different temperatures. The polyCBAA grafting was done via atom-transfer radical polymerization (ATRP) with varying film thicknesses. The objective is to create a surface that experiences "zero" protein adsorption from complex undiluted human blood serum and plasma. Results show that protein adsorption from undiluted human blood serum, plasma, and aged serum on the polyCBAA-grafted surface is undetectable at both 25 and 37 degrees C by a surface plasmon resonance (SPR) sensor. This was achieved with a film thickness of approximately 21 nm. Furthermore, it is demonstrated that the polyCBAA surfaces after antibody immobilization maintain undetectable protein adsorption from undiluted human blood serum. This is the first time that an effective nonfouling material suitable for applications in complex blood media has been demonstrated.
由于人血清和血浆在材料表面具有很强的非特异性吸附，这给与血液接触的装置和植入材料带来了巨大挑战。在本研究中，我们研究了在不同温度下，单一蛋白质溶液以及诸如未稀释的人血清和血浆等复杂介质中的蛋白质在聚（羧酸甜菜碱丙烯酰胺）（polyCBAA）接枝表面的非特异性吸附情况。通过原子转移自由基聚合（ATRP）实现了不同膜厚的polyCBAA接枝。目标是创造一种表面，使其在未稀释的人血清和血浆中实现“零”蛋白质吸附。结果表明，表面等离子体共振（SPR）传感器在25℃和37℃下均未检测到未稀释的人血清、血浆及老化血清中的蛋白质在polyCBAA接枝表面的吸附。这一结果在约21nm的膜厚下得以实现。此外，研究表明，固定抗体后的polyCBAA表面在未稀释的人血清中仍保持不可检测的蛋白质吸附。这是首次证明一种适用于复杂血液介质应用的有效抗污染材料。
